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首页> 外文期刊>Pharmacognosy Research >Evaluation of Antiulcer and Antioxidant Activity of Barleria gibsoni Dalz. Leaves
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Evaluation of Antiulcer and Antioxidant Activity of Barleria gibsoni Dalz. Leaves

机译：吉布勒氏菌（Barleria gibsoni Dalz）的抗溃疡和抗氧化活性评估。树叶

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Background: Peptic ulcer is a digestive disorder most commonly found in clinical practice. Given the many side effects of modern medicine, the initial acquisition of fewer side effects, and medication of indigenous drugs, it should be considered as a better alternative for the treatment of peptic ulcer. Objective: To assess antiulcer and antioxidant activity of ethanol extract of Barleria gibsoni (EBG) Dalz. leaves in ulcer-induced rats and in vitro antioxidants method, respectively. Materials and Methods: Ethanol EBG was screened for antiulcer activity in pylorus ligation-induced ulcer models in Wistar rats. In vitro antioxidant activity of the extracts was tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) radical scavenging activity. Total phenol and flavonoid content in the extracts were determined spectrophotometrically. Results: Oral administration of ethanol extract of leaves at doses of 250, 500 mg/kg p.o. reduced significant gastric lesions induced by pylorus ligation-induced ulcer as compared to standard omeprazole (20 mg/kg p.o.). The IC50 values were found to be 150 μg/mL in leaves extract. The ethanol extracts showed good antioxidant capacity in DPPH radical scavenging assay and NO radical scavenging activity when compared to standard. The total phenolic content using Folin–Ciocalteu reagent estimated in 1 mg of leaves extracts was 368 μg and 481 μg with gallic acid equivalent and also the total flavonoid content found to be 240 and 410 μg, respectively, with quercetin equivalence. Conclusion: These findings suggest that the leaves of B. gibsoni possessed antiulcer potential and antioxidant compared to standard. This is the first ever report of antiulcer and antioxidant activities in B. gibsoni ( Acanthaceae ). SUMMARY In vivo antiulcer and in vitro antioxidant activity of Barleria gibsoni was evaluated. Soxhelt extraction was carried out and extracts were subjected to qualitative phytochemical analysis. Extract obtained by Soxhlation showed higher total phenolic and flavonoid contents. EBG showed DPPH and Nitric oxide scavenging activity indicating its strong antioxidant potential. On pylorus ligation-accumulated secretions and the related ulcers confirm gastric acid output to be the basic cause of gastric ulcers. Ethanol extract of leaves attenuated the gastric volume, free acidity, total acidity and ulcer index thus showing the anti-secretory mechanism. The results of the histopathological investigation of Barleria gibsoni leaves for antiulcer effects using pylorus ligation induced ulcer model in rats laid credence to traditional use of the plant leaves in ulcer treatment. The ethanol extract of leaves demonstrated increase in percentage preventive index compared to omeprazole respectively. From the present study results reveals the antiulcer activity of ethanol extract leaves which is comparable to that of Omeprazole. Abbreviations Used : EBG: Ethanol extract prepared from the leaves of B. gibsoni , ROS: Reactive Oxygen Species, DPPH: 2, 2-diphenyl-1-picrylhydrazyl, NO: Nitric Oxide, IC50: The half maximal inhibitory concentration, m: mg, DNA: deoxyribonucleic acid, GAE: Gallic acid equivalence, AlCl3: Aluminium chloride Key words: Acanthaceae , Barleria gibsoni , gallic acid, L-ascorbic acid, pylorus ligation-induced ulcer model, quercetin.
机译：背景：消化性溃疡是一种在临床实践中最常见的消化系统疾病。鉴于现代医学的许多副作用，初期获得的副作用较少以及使用本土药物治疗，应将其视为治疗消化性溃疡的更好选择。目的：评估巴氏菌（Dalz）的乙醇提取物的抗溃疡和抗氧化活性。分别在溃疡诱导的大鼠叶片和体外抗氧化剂方法中使用。材料和方法：在Wistar大鼠幽门结扎诱导的溃疡模型中筛选乙醇EBG的抗溃疡活性。使用2，2-二苯基-1-吡啶并肼基（DPPH），一氧化氮（NO）自由基清除活性测试了提取物的体外抗氧化活性。分光光度法测定提取物中的总酚和类黄酮含量。结果：口服给予叶片乙醇提取物，剂量为250，500 mg / kgp.o。与标准的奥美拉唑（20 mg / kg p.o.）相比，可减少因幽门结扎引起的溃疡引起的明显胃部病变。叶片提取物中的IC 50 值为150μg/ mL。与标准品相比，乙醇提取物在DPPH自由基清除试验中显示出良好的抗氧化能力，并具有NO清除自由基的活性。使用Folin–Ciocalteu试剂估算的1 mg叶片提取物中的总酚含量为368μg和481μg（与没食子酸相当），总黄酮含量分别为240和410μg（与槲皮素相当）。结论：这些发现表明，长双歧杆菌的叶子具有抗溃疡潜力和抗氧化剂。这是关于吉布森牛鞭草（Acanthaceae）抗溃疡和抗氧化活性的首次报道。发明内容评估了长发barleria gibsoni的体内抗溃疡和体外抗氧化活性。进行索氏提取，并对提取物进行定性植物化学分析。通过Soxhlation获得的提取物显示出较高的总酚和类黄酮含量。 EBG表现出DPPH和一氧化氮清除活性，表明其强大的抗氧化潜能。在幽门结扎积累的分泌物和相关的溃疡上证实胃酸输出是胃溃疡的根本原因。叶子的乙醇提取物减弱了胃的体积，游离酸度，总酸度和溃疡指数，从而显示出抗分泌的机制。使用幽门结扎诱导的大鼠溃疡模型对大叶青霉叶片抗溃疡作用的组织病理学研究结果证实了传统方法将植物叶片用于溃疡治疗。叶片的乙醇提取物与奥美拉唑相比，分别具有更高的预防指数。从目前的研究结果表明，乙醇提取物叶的抗溃疡活性与奥美拉唑相当。所用缩写：EBG：从长双歧杆菌叶中提取的乙醇提取物，ROS：活性氧，DPPH：2，2-二苯基-1-吡啶并肼，NO：一氧化氮，IC 50 ：最大半数抑制浓度，m：mg，DNA：脱氧核糖核酸，GAE：没食子酸当量，AlCl 3 ：氯化铝关键词：棘皮科，Barleria gibsoni，没食子酸，L-抗坏血酸，幽门结扎诱导的溃疡模型槲皮素。

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