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首页> 外文期刊>Pharmacognosy Research >Probing Regenerative Potential of Moringa oleifera Aqueous Extracts Using In vitro Cellular Assays
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Probing Regenerative Potential of Moringa oleifera Aqueous Extracts Using In vitro Cellular Assays

机译：辣木水提取物的再生潜能的体外细胞分析

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Background: Molecules stimulating regeneration and proliferation of cells are of significance in combating ailments caused due to tissue injury, inflammation, and degenerative disorders. Moringa oleifera is one of the most valued food plants having the profile of important nutrients and impressive range of medicinal uses. Objective: To evaluate the potential of M. oleifera aqueous leaf and flower extracts to promote the proliferation of cells and explore their effect on cancer cell lines for assessment of safety. Materials and Methods: Aqueous leaf and flower extracts of M. oleifera were investigated for effect on rat-derived primary fibroblast, mesenchymal stem cells (MSCs), and cancer cell lines using cell proliferation assay. They were also tested and compared for wound healing, angiogenesis, and hepatoprotective effect using in vitro assays. Results: Statistically significant increase in the proliferation of primary rat fibroblast, MSCs, and angiogenesis was observed after treatment with aqueous flower extract. The aqueous leaf extract determined a comparatively moderate increment in the proliferation of MSCs and angiogenesis. It however showed prominent cytotoxicity to cancer cell lines and a significant hepatoprotective effect. Conclusion: A very clear difference in response of the two extracts to different types of cells was detected in this study. The aqueous flower extract exhibited a higher potential to stimulate cell proliferation while not exerting the same effect on cancer cell lines. The leaf extract on the other hand, had a prominent antitumor and hepatoptotective effects. SUMMARY Moringa oleifera flower extract showed significant ability to promote proliferation of rat fibroblast and mesenchymal stem cells. The extract also had prominent angiogenic and hepatoprotective effects. The extract did not influence proliferation of cancer cell lines indicating its safety for human consumption and use in pharmaceuticals. The Moringa oleifera leaf extract showed relatively less potential to stimulate cells but had prominent cytotoxic effect on cancer cell lines. Abbreviations Used : ALT: Alanine transaminase, AST: Asparatate amino transferase, ATCC: American type culture collection, BMMSC: Bone marrow mesenchymal stem cells (used in this paper), CAM: Chick chorioallantoic membrane, CCl4: Carbon tetra chloride, DMEM: Dulbecco's modified Eagle medium, DMSO: Dimethyl sulfoxide, EDTA: Ethylene diamine tetraacetic acid, HBL 100: Human breast epithelial cell line, Mcf-7: Human breast adenocarcinoma cell line, aMEM: Minimum Essential Medium Eagle alpha modification, MOF: Moringa oleifera aqueous flower extract (used in this paper), MOL: Moringa oleifera aqueos leaf extract (Used in this paper), OD: Optical density, PBS: Phosphate buffered saline Key words: Aqueous, cytotoxicity, fibroblast, in vitro , viability.
机译：背景：刺激细胞再生和增殖的分子在对抗因组织损伤，炎症和变性疾病引起的疾病中具有重要意义。辣木是最重要的食用植物之一，具有重要的营养成分和令人印象深刻的药用范围。目的：评价油茶（M.oleifera）水叶和花提取物促进细胞增殖的潜力，并探讨其对癌细胞系的作用，以评估安全性。材料和方法：使用细胞增殖试验研究了油茶的叶和花提取物对大鼠来源的原代成纤维细胞，间充质干细胞（MSCs）和癌细胞系的影响。还使用体外试验对它们进行了测试并比较了伤口愈合，血管生成和肝保护作用。结果：用水性花提取物处理后，观察到原代大鼠成纤维细胞，MSCs的增殖和血管生成的统计学显着增加。含水叶提取物确定了MSCs增殖和血管生成的相对中等的增量。然而，它显示出对癌细胞系的显着细胞毒性和显着的肝保护作用。结论：本研究发现两种提取物对不同类型细胞的反应存在非常明显的差异。水性花提取物表现出更高的刺激细胞增殖的潜力，而对癌细胞系没有发挥相同的作用。另一方面，叶提取物具有显着的抗肿瘤和肝保护作用。发明内容辣木花提取物显示出显着的能力促进大鼠成纤维细胞和间充质干细胞的增殖。该提取物还具有显着的血管生成和肝保护作用。提取物不影响癌细胞系的增殖，表明其对人类食用和用于药物的安全性。辣木叶提取物显示出相对较小的刺激细胞潜力，但对癌细胞系具有显着的细胞毒性作用。所用缩写：ALT：丙氨酸转氨酶，AST：天冬氨酸氨基转移酶，ATCC：美国典型培养物保藏中心，BMMSC：骨髓间充质干细胞（本文中使用），CAM：雏鸡尿囊膜，CCl4：四氯化碳，DMEM：Dulbecco's改良的Eagle培养基，DMSO：二甲基亚砜，EDTA：乙二胺四乙酸，HBL 100：人乳腺上皮细胞系，Mcf-7：人乳腺腺癌细胞系，aMEM：最低必需培养基Eagle alpha修饰，MOF：辣木含水花提取物（本文中使用），MOL：辣木叶片提取物（本文中使用），OD：光密度，PBS：磷酸盐缓冲盐水关键词：水溶液，细胞毒性，成纤维细胞，体外，存活力。

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