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首页> 外文期刊>Pharmacognosy Research >Assessment of Anti-Influenza Activity and Hemagglutination Inhibition of Plumbago indica and Allium sativum Extracts
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Assessment of Anti-Influenza Activity and Hemagglutination Inhibition of Plumbago indica and Allium sativum Extracts

机译：lum和大蒜提取物的抗流感活性和血凝抑制作用的评估

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Background: Human influenza is a seasonal disease associated with significant morbidity and mortality. Anti-flu ayurvedic/herbal medicines have played a significant role in fighting the virus pandemic. Plumbagin and allicin are commonly used ingredients in many therapeutic remedies, either alone or in conjunction with other natural substances. Evidence suggests that these extracts are associated with a variety of pharmacological activities. Objective: To evaluate anti-influenza activity from Plumbago indica and Allium sativum extract against Influenza A (H1N1)pdm09. Materials and Methods: Different extraction procedures were used to isolate the active ingredient in the solvent system, and quantitative HPLTC confirms the presence of plumbagin and allicin. The cytotoxicity was carried out on Madin-Darby Canine kidney cells, and the 50% cytotoxic concentration (CC50) values were below 20 mg/mL for both plant extracts. To assess the anti-influenza activity, two assays were employed, simultaneous and posttreatment assay. Results: A. sativum methanolic and ethanolic extracts showed only 14% reduction in hemagglutination in contrast to P. indica which exhibited 100% reduction in both simultaneous and posttreatment assay at concentrations of 10 mg/mL, 5 mg/mL, and 1 mg/mL. Conclusions: Our results suggest that P. indica extracts are good candidates for anti-influenza therapy and should be used in medical treatment after further research. SUMMARY The search for natural antiviral compounds from plants is a promising approach in the development of new therapeutic agents. In the past century, several scientific efforts have been directed toward identifying phytochemicals capable of inhibiting virus. Knowledge of ethnopharmacology can lead to new bioactive plant compounds suitable for drug discovery and development. Macromolecular docking studies provides most detailed possible view of drug-receptor interaction where the structure of drug is designed based on its fit to three dimensional structures of receptor site rather than by analogy to other active structures or random leads. Our previous studies indicate that Allicin sand Plumbagin could be used as the potent multi drug targets against the Neuraminidase, Hemagglutinin and M2 protein channel of influenza A (H1N1) pdm09. This in-vittro study has shown that P. indica L. and A. sativum extracts can inhibit influenza A (H1N1)pdm09 virus by inhibiting viral nucleoprotein synthesis and polymerase activity.
机译：背景：人流感是与明显的发病率和死亡率相关的季节性疾病。抗流感阿育吠陀/草药在对抗病毒大流行中发挥了重要作用。 Plumbagin和大蒜素是许多治疗药物中常用的成分，无论是单独使用还是与其他天然物质结合使用。有证据表明这些提取物与多种药理活性有关。目的：评价P子和大蒜提取物对甲型流感（H1N1）pdm09的抗流感活性。材料和方法：采用不同的提取程序分离溶剂系统中的活性成分，定量HPLTC证实存在铅皮和大蒜素。对Madin-Darby犬肾细胞进行了细胞毒性试验，两种植物提取物的50％细胞毒性浓度（CC 50 ）值均低于20 mg / mL。为了评估抗流感活性，采用了两种测定，即同时测定和后处理测定。结果：与甲醇假单胞菌相比，sa菜甲醇和乙醇提取物的血凝集减少仅14％，而在浓度为10 mg / mL，5 mg / mL和1 mg / mL的同时处理和后处理中assay菜均表现出100％的减少。毫升结论：我们的结果表明，印度P提取物是抗流感治疗的良好候选者，应在进一步研究后用于医学治疗。发明内容从植物中寻找天然抗病毒化合物是开发新治疗剂的有前途的方法。在过去的一个世纪中，已经进行了一些科学努力来鉴定能够抑制病毒的植物化学物质。民族药理学知识可以导致适用于药物发现和开发的新型生物活性植物化合物。高分子对接研究提供了最详细的药物与受体相互作用的观点，其中药物的结构是根据其对受体位点的三维结构的适应性来设计的，而不是通过与其他活性结构或随机前导的类比来设计的。我们以前的研究表明，大蒜素沙栗可以用作抗A型流感（H1N1）pdm09的神经氨酸酶，血凝素和M2蛋白通道的有效多药靶标。这项体内研究表明，印度假单胞菌和sa菜提取物可以通过抑制病毒核蛋白的合成和聚合酶活性来抑制甲型流感（H1N1）pdm09病毒。

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