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首页> 外文期刊>Plant Protection Science >Wheat dwarf virus infectious clones allow to infect wheat and Triticum monococcum plants
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Wheat dwarf virus infectious clones allow to infect wheat and Triticum monococcum plants

机译：小麦矮病毒感染性克隆可感染小麦和小麦

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We constructed Wheat dwarf virus (WDV) infectious clones in the bacterial plasmids pUC18 and pIPKb002 and tested their ability to inoculate plants using Bio-Rad Helios Gene Gun biolistic inoculation method and Agrobacterium tumefaciens agroinoculation method, and we then compared them with the natural inoculation method via viruliferous P. alienus. Infected plants were generated using both infectious clones, whereas the agroinoculation method was able to produce strong systemic infection in all three tested cultivars of wheat and Triticum monococcum, comparable to plants inoculated by viruliferous P. alienus. Infection was confirmed by DAS-ELISA, and WDV titres were quantified using qPCR. The levels of remaining bacterial plasmid DNA were also confirmed to be zero.
机译：我们在细菌质粒pUC18和pIPKb002中构建了小麦矮病毒（WDV）感染性克隆，并使用Bio-Rad Helios Gene Gun生物弹接种法和根癌农杆菌农杆菌接种法测试了它们对植物的接种能力，然后将其与自然接种法进行了比较。通过有毒的P. Alienus。使用两个感染性克隆均产生了受感染的植物，而农杆菌接种方法能够在小麦和小麦的所有三个测试品种中产生强烈的全身感染，这与有毒的外来疟原虫接种的植物相当。通过DAS-ELISA确认感染，并使用qPCR定量WDV滴度。剩余的细菌质粒DNA的水平也被确认为零。

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