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首页> 外文期刊>Plant Breeding and Seed Science >Molecular diversity and phylogeny of Triticum-Aegilops species possessing D genome revealed by SSR and ISSR markers
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Molecular diversity and phylogeny of Triticum-Aegilops species possessing D genome revealed by SSR and ISSR markers

机译：SSR和ISSR标记揭示具有D基因组的普通小麦物种的分子多样性和系统发育

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The aim of this study is investigation the applicability of SSR and ISSR markers in evaluating the genetic relationships in twenty accessions of Aegilops and Triticum species with D genome in different ploidy levels. Totally, 119 bands and 46 alleles were detected using ten primers for ISSR and SSR markers, respectively. Polymorphism Information Content values for all primers ranged from 0.345 to 0.375 with an average of 0.367 for SSR, and varied from 0.29 to 0.44 with the average 0.37 for ISSR marker. Analysis of molecular variance (AMOVA) revealed that 81% (ISSR) and 84% (SSR) of variability was partitioned among individuals within populations. Comparing the genetic diversity of Aegilops and Triticum accessions, based on genetic parameters, shows that genetic variation of Ae. crassa and Ae. tauschii species are higher than other species, especially in terms of Nei’s gene diversity. Cluster analysis, based on both markers, separated total accessions in three groups. However, classification based on SSR marker data was not conformed to classification according to ISSR marker data. Principal co-ordinate analysis (PCoA) for SSR and ISSR data showed that, the first two components clarified 53.48% and 49.91% of the total variation, respectively. This analysis (PCoA), also, indicated consistent patterns of genetic relationships for ISSR data sets, however, the grouping of accessions was not completely accorded to their own geographical origins. Consequently, a high level of genetic diversity was revealed from the accessions sampled from different eco-geographical regions of Iran.
机译：这项研究的目的是调查SSR和ISSR标记在评估不同倍性水平上20个带有D基因组的埃及盾和小麦种的遗传关系中的适用性。总共使用10条ISSR和SSR标记引物检测到119个条带和46个等位基因。多态性信息所有引物的含量值范围从0.345到0.375，SSR平均为0.367，从0.29到0.44，ISSR标记平均为0.37。分子方差分析（AMOVA）显示，人口中的个体之间存在81％（ISSR）和84％（SSR）的变异性。根据遗传参数比较埃及斑节麦和小麦种的遗传多样性，可以看出Ae的遗传变异。 crassa和Ae。 tauschii种比其他种更高，特别是在Nei的基因多样性方面。基于这两个标记的聚类分析将总种分为三组。然而，基于SSR标记数据的分类不符合根据ISSR标记数据的分类。 SSR和ISSR数据的主坐标分析（PCoA）表明，前两个成分分别澄清了总变异的53.48％和49.91％。该分析（PCoA）也表明ISSR数据集的遗传关系具有一致的模式，但是，种质的分组并不完全符合其自身的地理起源。因此，从伊朗不同生态地理区域取样的种质揭示出高度的遗传多样性。

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