...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Biotechnology Journal >Soybean WRKY?￠????type transcription factor genes, GmWRKY13, GmWRKY21 , and GmWRKY54 , confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants
【24h】

Soybean WRKY?￠????type transcription factor genes, GmWRKY13, GmWRKY21 , and GmWRKY54 , confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants

机译：大豆WRKY ????型转录因子基因GmWRKY13，GmWRKY21和GmWRKY54使转基因拟南芥植物对非生物胁迫具有不同的耐受性

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

WRKY?￠????type transcription factors have multiple roles in the plant defence response and developmental processes. Their roles in the abiotic stress response remain obscure. In this study, 64 GmWRKY genes from soybean were identified, and were found to be differentially expressed under abiotic stresses. Nine GmWRKY proteins were tested for their transcription activation in the yeast assay system, and five showed such ability. In a DNA?￠????binding assay, three proteins (GmWRKY13, GmWRKY27 and GmWRKY54) with a conserved WRKYGQK sequence in their DNA?￠????binding domain could bind to the W?￠????box (TTGAC). However, GmWRKY6 and GmWRKY21, with an altered sequence WRKYGKK, lost the ability to bind to the W?￠????box. The function of three stress?￠????induced genes, GmWRKY13 , GmWRKY21 and GmWRKY54 , was further investigated using a transgenic approach. GmWRKY21?￠???? transgenic Arabidopsis plants were tolerant to cold stress, whereas GmWRKY54 conferred salt and drought tolerance, possibly through the regulation of DREB2A and STZ/Zat10 . Transgenic plants over?￠????expressing GmWRKY13 showed increased sensitivity to salt and mannitol stress, but decreased sensitivity to abscisic acid, when compared with wild?￠????type plants. In addition, GmWRKY13 ?￠????transgenic plants showed an increase in lateral roots. These results indicate that the three GmWRKY genes play differential roles in abiotic stress tolerance, and that GmWRKY13 may function in both lateral root development and the abiotic stress response.
机译：WRKYβ型转录因子在植物防御反应和发育过程中具有多种作用。它们在非生物应激反应中的作用仍然不清楚。在这项研究中，从大豆中鉴定出64个GmWRKY基因，并发现它们在非生物胁迫下差异表达。在酵母测定系统中测试了九种GmWRKY蛋白的转录激活，其中五种显示了这种能力。在DNA结合测定中，三种在其DNA结合域中具有保守WRKYGQK序列的蛋白质（GmWRKY13，GmWRKY27和GmWRKY54）可以结合到W？ TTGAC）。然而，具有改变的序列WRKYGKK的GmWRKY6和GmWRKY21失去了与Wβ盒的结合的能力。使用转基因方法进一步研究了三种胁迫诱导的基因GmWRKY13，GmWRKY21和GmWRKY54的功能。 GmWRKY21？￠ ????转基因拟南芥植物耐受冷胁迫，而GmWRKY54可能通过调节DREB2A和STZ / Zat10赋予盐和干旱耐受性。与野生型植物相比，过量表达GmWRKY13的转基因植物显示出对盐和甘露醇胁迫的敏感性增加，但是对脱落酸的敏感性降低。另外，GmWRKY13β转基因植物显示出侧根增加。这些结果表明，三个GmWRKY基因在非生物胁迫耐受性中起着不同的作用，并且GmWRKY13可能在侧根发育和非生物胁迫响应中起作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号