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Rhizobium japonicum as a biocontrol agent of soybean root rot disease caused by Fusarium solani and Macrophomina phaseolina

机译:日本根瘤菌作为茄根镰刀菌和菜豆巨噬菌引起的大豆根腐病的生物防治剂

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摘要

The activity of Rhizobium japonicum against the soil-borne pathogens Fusarium solani and Macrophomina phaseolina as causative agents of soybean root rot disease in both culture medium and soil was evaluated. Rhizobial culture filtrate caused an inhibition of the fungal radial growth of Fusarium solani and Macrophomina phaseolina on potato dextrose agar medium amended with the filtrate compared with control. The addition of rhizobial culture suspension to the soil contaminated by the two pathogens, Fusarium solani and Macrophomina phaseolina and their interaction, in pots, improved seed germination percentages and reduced the root rot disease index significantly. The sowing of rhizobial coated seeds in soil contaminated by Fusarium solani and Macrophomina phaseolina separately and in combination, in the field, increased seed germination significantly and induced a high reduction in disease severity for the same previous combination under field conditions. These results indicate that rhizobia could be an important element in root rot disease management.
机译:评价了日本根瘤菌对土壤传播的病原菌镰刀镰刀菌和菜豆大单胞菌作为大豆根腐病病原体在培养基和土壤中的活性。与对照相比,根瘤菌培养滤液对用该滤液改良的马铃薯葡萄糖琼脂培养基对茄形镰刀菌和菜豆大孢子菌的真菌径向生长具有抑制作用。将根瘤菌培养悬浮液添加到被两种病原菌镰刀镰刀菌和菜豆大环孢菌污染的土壤中,以及它们在花盆中的相互作用,可提高种子发芽率并显着降低根腐病指数。在田间条件下,在相同条件下,在田间单独施用镰刀镰刀菌和菜豆(Macrophomina phaseolina)污染的土壤中,根瘤菌包衣种子的播种显着增加了种子的发芽率,并大大降低了病害的严重程度。这些结果表明,根瘤菌可能是根腐病防治中的重要因素。

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