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Studies on the Technique of Tissue Culture and Rapid Propagation of Bulbils From Lilium regale

机译：帝王百合的组织培养及快速繁殖技术研究

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Bulbils of Lilium regale were used as explants. Adventitious bud induction and the proliferation were studied. The results showed that the regeneration ability of outer layer scales of Lilium regale was better than the middle ones, which was better than the inner ones. The adaxial side of Lilium regale bulbils in contact with medium upward could produce more and stronger adventitious buds than the adaxial side downward. For outer and middle layer scales, the optimum medium for adventitious bud induction was MS +KT 1.0 mg L-1 + NAA 0.2 mg L-1 + CH 500 mg L-1. The adventitious bud induction rate was 100.0% and each explant could produce 12.7 adventitious buds. For the inner ones, the appropriate medium for adventitious bud induction was MS + ZT 1.5 mg L-1 + CH 500 mg L-1 or MS + KT 1.5 mg L-1 + CH 500 mg L-1. The medium MS + KT 0.5 mg L-1 + NAA 0.1 mg L-1 + CH 500 mg L-1 could make proliferation and rooting completed synchronously. The multiplication coefficient was 7.3.
机译：普通百合的外耳用作外植体。研究了不定芽的诱导和增殖。结果表明，普通百合外层鳞片的再生能力强于中间鳞片，而内层鳞片的再生能力强于内层鳞片。与中间向上接触的帝王百合球茎的近侧比向下的正侧可以产生更多，更强的不定芽。对于外层和中层鳞片，不定芽诱导的最佳培养基为MS + KT 1.0 mg L-1 + NAA 0.2 mg L-1 + CH 500 mg L-1。不定芽诱导率为100.0％，每个外植体可产生12.7个不定芽。对于内部培养基，不定芽诱导的合适培养基为MS + ZT 1.5 mg L-1 + CH 500 mg L-1或MS + KT 1.5 mg L-1 + CH 500 mg L-1。 MS + KT 0.5 mg L-1 + NAA 0.1 mg L-1 + CH 500 mg L-1可使繁殖和生根同步完成。乘法系数为7.3。

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