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Production of astragaloside and flavones from adventitious root cultures of Astragalus membranaceus var. mongholicus

机译:从黄芪不定根培养物中生产黄芪甲苷和黄酮。蒙古人

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Many plants contain various secondary metabolites that have medicinal value. Their content varies across plant parts. We carried out in vitro adventitious root induction from leaf explants of Huang-qi (Astragalus membranaceus) using different nutrient media supplemented with various plant hormones. The level of astragaloside and flavones were analyzed from the adventitious roots of A. membranaceus grown under these different media conditions. Among the different media and plant hormones, Murashige and Skoog medium supplemented with 1.0 mg/L 1-naphthaleneacetic acid resulted in the greatest degree of adventitious root induction. The highest concentration of calycosin (11.7 μg/g dry weight), calycosin-7-glu (13.3 μg/g dry weight), and glucoraphanin (241.9 μg/g dry weight) were observed in roots grown in half-strength Schenk & Hildebrandt mineral solution, half-strength Gamborg’s B5 medium, and full-strength Gamborg’s B5 media, respectively.
机译:许多植物含有各种具有药用价值的次级代谢产物。它们的含量因植物部位而异。我们利用补充了多种植物激素的不同营养培养基,从黄芪(黄芪)的外植体中进行了不定根的体外诱导。分析了在这些不同培养基条件下生长的膜曲霉不定根中黄芪甲苷和黄酮的含量。在不同的培养基和植物激素中,添加1.0 mg / L 1-萘乙酸的Murashige和Skoog培养基导致最大程度的不定根诱导。在半强度的Schenk&Hildebrandt种植的根中,观察到最高浓度的树胶蛋白(11.7μg/ g干重),7糖蛋白-glu(13.3μg/ g干重)和糖尿素(241.9μg/ g干重)。矿物溶液,半强度Gamborg的B5培养基和全强度Gamborg的B5培养基。

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