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首页> 外文期刊>PLoS Biology >The Bacterial Effector HopX1 Targets JAZ Transcriptional Repressors to Activate Jasmonate Signaling and Promote Infection in Arabidopsis
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The Bacterial Effector HopX1 Targets JAZ Transcriptional Repressors to Activate Jasmonate Signaling and Promote Infection in Arabidopsis

机译：细菌效应器HopX1靶向JAZ转录阻遏物，以激活茉莉酸信号并促进拟南芥中的感染。

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Pathogenicity of Pseudomonas syringae is dependent on a type III secretion system, which secretes a suite of virulence effector proteins into the host cytoplasm, and the production of a number of toxins such as coronatine (COR), which is a mimic of the plant hormone jasmonate-isoleuce (JA-Ile). Inside the plant cell, effectors target host molecules to subvert the host cell physiology and disrupt defenses. However, despite the fact that elucidating effector action is essential to understanding bacterial pathogenesis, the molecular function and host targets of the vast majority of effectors remain largely unknown. Here, we found that effector HopX1 from Pseudomonas syringae pv. tabaci (Pta) 11528, a strain that does not produce COR, interacts with and promotes the degradation of JAZ proteins, a key family of JA-repressors. We show that hopX1 encodes a cysteine protease, activity that is required for degradation of JAZs by HopX1. HopX1 associates with JAZ proteins through its central ZIM domain and degradation occurs in a COI1-independent manner. Moreover, ectopic expression of HopX1 in Arabidopsis induces the expression of JA-dependent genes, represses salicylic acid (SA)-induced markers, and complements the growth of a COR-deficient P. syringae pv. tomato (Pto) DC3000 strain during natural bacterial infections. Furthermore, HopX1 promoted susceptibility when delivered by the natural type III secretion system, to a similar extent as the addition of COR, and this effect was dependent on its catalytic activity. Altogether, our results indicate that JAZ proteins are direct targets of bacterial effectors to promote activation of JA-induced defenses and susceptibility in Arabidopsis. HopX1 illustrates a paradigm of an alternative evolutionary solution to COR with similar physiological outcome.
机译：丁香假单胞菌的致病性取决于III型分泌系统，该系统将一系列毒力效应蛋白分泌到宿主细胞质中，并产生许多毒素，例如可乐定（COR），它是植物激素茉莉的模拟物。 -isoleuce（JA-Ile）。在植物细胞内部，效应子靶向宿主分子，以破坏宿主细胞的生理机能并破坏防御力。然而，尽管阐明效应子的作用对于理解细菌的发病机理是必不可少的，但是绝大多数效应子的分子功能和宿主靶点仍然是未知的。在这里，我们发现了丁香假单胞菌pv的效应子HopX1。烟粉虱（Pta）11528，不产生COR的菌株，与JA阻遏物的重要家族JAZ蛋白相互作用并促进其降解。我们显示，hopX1编码一个半胱氨酸蛋白酶，HopX1降解JAZs所需的活性。 HopX1通过其中央ZIM结构域与JAZ蛋白缔合，降解以COI1独立的方式发生。此外，HopX1在拟南芥中的异位表达诱导JA依赖性基因的表达，抑制水杨酸（SA）诱导的标记，并补充COR缺失的丁香假单胞菌pv的生长。天然细菌感染期间的番茄（Pto）DC3000菌株。此外，HopX1在通过天然III型分泌系统传递时提高了敏感性，其程度与添加COR相似，并且这种作用取决于其催化活性。总而言之，我们的结果表明JAZ蛋白是细菌效应子的直接靶标，以促进JA诱导的拟南芥防御和敏感性的激活。 HopX1说明了具有相似生理结果的COR替代进化解决方案的范例。

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