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首页> 外文期刊>PLoS Genetics >Sequential Assembly of Centromeric Proteins in Male Mouse Meiosis
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Sequential Assembly of Centromeric Proteins in Male Mouse Meiosis

机译：雄性小鼠减数分裂中着丝粒蛋白的顺序组装

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The assembly of the mitotic centromere has been extensively studied in recent years, revealing the sequence and regulation of protein loading to this chromosome domain. However, few studies have analyzed centromere assembly during mammalian meiosis. This study specifically targets this approach on mouse spermatocytes. We have found that during prophase I, the proteins of the chromosomal passenger complex Borealin, INCENP, and Aurora-B load sequentially to the inner centromere before Shugoshin 2 and MCAK. The last proteins to be assembled are the outer kinetochore proteins BubR1 and CENP-E. All these proteins are not detected at the centromere during anaphase/telophase I and are then reloaded during interkinesis. The loading sequence of the analyzed proteins is similar during prophase I and interkinesis. These findings demonstrate that the interkinesis stage, regularly overlooked, is essential for centromere and kinetochore maturation and reorganization previous to the second meiotic division. We also demonstrate that Shugoshin 2 is necessary for the loading of MCAK at the inner centromere, but is dispensable for the loading of the outer kinetochore proteins BubR1 and CENP-E.
机译：近年来，对有丝分裂着丝粒的组装进行了广泛的研究，揭示了该染色体结构域的蛋白质负载序列和调控。但是，很少有研究分析哺乳动物减数分裂过程中的着丝粒组装。这项研究专门针对小鼠精母细胞这种方法。我们已经发现，在前期I期间，染色体乘客复合体Borealin，INSENP和Aurora-B的蛋白质先后在Shugoshin 2和MCAK之前加载到内部着丝粒。最后要组装的蛋白质是外部动粒蛋白BubR1和CENP-E。在后期I /末期I的着丝粒中未检测到所有这些蛋白，然后在间质分离期间重新加载。在前期I和动因之间，被分析蛋白质的加载顺序相似。这些发现表明，经常被忽略的过渡间阶段对于第二个减数分裂分裂之前的着丝粒和线粒体的成熟和重组至关重要。我们还证明了Shugoshin 2对于内着丝粒MCAK的加载是必需的，但对于外着丝粒蛋白BubR1和CENP-E的加载是必不可少的。

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