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首页> 外文期刊>PLoS One >Goat Uterine DBA+ Leukocytes Differentiation and Cytokines Expression Respond Differently to Cloned versus Fertilized Embryos
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Goat Uterine DBA+ Leukocytes Differentiation and Cytokines Expression Respond Differently to Cloned versus Fertilized Embryos

机译：山羊子宫DBA +白细胞分化和细胞因子表达对克隆和受精胚胎的反应不同

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High rate of fetal mortality in ruminant somatic cell nuclear transfer (SCNT) pregnancies is due, at least in part, to immune-mediated abortion of fetuses. In the present study, goat uterine leukocytes were isolated by Dolichos biflorus agglutinin (DBA) coated magnetic beads, and with majority being were CD56+CD16- in phenotype with low levels of perforin and Granzyme B expression. The responses of the isolated cells to SCNT and in vitro fertilization (IVF) embryos conditioned mediums containing hormone steroids were compared by measuring their phenotype and cytokines expression. The results showed there was a 2-fold increase in the numbers of isolated uterine leukocytes after incubation with different conditioned mediums for 120 h. However, significantly lower percentage and absolute numbers of uterine CD56+CD16- leukocytes incubated with SCNT conditioned mediums were detected as compared with those incubated with IVF conditioned mediums (P 0.05). The group treated with progesterone (P4) or the combination of P4 and 17β-estradiol (E2) were associated with significantly higher percentage and absolute numbers of CD56+CD16- cells as compared with those treated with E2 alone (P 0.05). Furthermore, in the presence of steroids, the isolated leukocytes incubated with SCNT conditioned mediums associated with greater levels of IFN-γ secretion and expression, as well as lesser levels of VEGF, as compared with those treated with IVF conditioned mediums (P 0.05). In conclusion, this study demonstrates that SCNT embryos have a profound effect on the phenotype expression of goat uterine DBA+ leukocytes, as well as the secretion and expression of IFN-γ and VEGF by these cells in vitro.
机译：反刍体细胞核移植（SCNT）妊娠中胎儿死亡率高，至少部分原因是免疫介导的胎儿流产。在本研究中，通过Dolichos biflorus凝集素（DBA）包被的磁珠分离了山羊子宫白细胞，其中大多数为CD56 + CD16-表型，穿孔素和粒酶B表达水平较低。通过测量它们的表型和细胞因子表达，比较了分离的细胞对SCNT的反应和体外受精（IVF）胚胎，其中含有激素类固醇。结果显示，与不同条件培养基孵育120小时后，分离出的子宫白细胞数量增加了2倍。但是，与用IVF条件培养基孵育的子宫CD56 + CD16-白细胞相比，检测到的子宫CD56 + CD16-白细胞的百分比和绝对数要低得多（P <0.05）。与单独使用E2治疗的组相比，使用孕酮（P4）或P4和17β-雌二醇的组合（E2）治疗的组与CD56 + CD16-细胞的百分比和绝对数目显着更高（P <0.05）。此外，与IVF条件培养基相比，在类固醇存在下，与SCNT条件培养基孵育分离的白细胞与更高水平的IFN-γ分泌和表达以及更低水平的VEGF相关（P <0.05） 。总之，这项研究表明，SCNT胚胎对山羊子宫DBA +白细胞的表型表达以及这些细胞在体外分泌和表达IFN-γ和VEGF具有深远的影响。

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