International Study to Evaluate PCR Methods for Detection of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients

Alejandro G. Schijman; Margarita Bisio; Liliana Orellana; Mariela Sued; Tomás Duffy; Ana M. Mejia Jaramillo; Carolina Cura; Frederic Auter; Vincent Veron; Yvonne Qvarnstrom; Stijn Deborggraeve; Gisely Hijar; Inés Zulantay; Raúl Horacio Lucero; Elsa Velazquez; Tatiana Tellez; Zunilda Sanchez Leon; Lucia Galv?o; Debbie Nolder; María Monje Rumi; José E. Levi; Juan D. Ramirez; Pilar Zorrilla; María Flores; Maria I. Jercic; Gladys Crisante; Néstor A?ez; Ana M. De Castro; Clara I. Gonzalez; Karla Acosta Viana; Pedro Yachelini; Faustino Torrico; Carlos Robello; Patricio Diosque; Omar Triana Chavez; Christine Aznar; Graciela Russomando; Philippe Büscher; Azzedine Assal; Felipe Guhl; Sergio Sosa Estani; Alexandre DaSilva; Constan?a Britto; Alejandro Luquetti; Janis Ladzins

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International Study to Evaluate PCR Methods for Detection of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients

机译：评价南美锥虫病患者血液中克氏锥虫DNA检测PCR方法的国际研究

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Background A century after its discovery, Chagas disease still represents a major neglected tropical threat. Accurate diagnostics tools as well as surrogate markers of parasitological response to treatment are research priorities in the field. The purpose of this study was to evaluate the performance of PCR methods in detection of Trypanosoma cruzi DNA by an external quality evaluation.

机译：背景恰加斯病发现一个世纪后，仍然代表着被忽视的主要热带威胁。准确的诊断工具以及寄生虫对治疗反应的替代指标是该领域的研究重点。这项研究的目的是通过外部质量评估来评估PCR方法在克氏锥虫DNA检测中的性能。

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《PLOS Neglected Tropical Diseases》 |2011年第1期|共13页
作者
Alejandro G. Schijman; Margarita Bisio; Liliana Orellana; Mariela Sued; Tomás Duffy; Ana M. Mejia Jaramillo; Carolina Cura; Frederic Auter; Vincent Veron; Yvonne Qvarnstrom; Stijn Deborggraeve; Gisely Hijar; Inés Zulantay; Raúl Horacio Lucero; Elsa Velazquez; Tatiana Tellez; Zunilda Sanchez Leon; Lucia Galv?o; Debbie Nolder; María Monje Rumi; José E. Levi; Juan D. Ramirez; Pilar Zorrilla; María Flores; Maria I. Jercic; Gladys Crisante; Néstor A?ez; Ana M. De Castro; Clara I. Gonzalez; Karla Acosta Viana; Pedro Yachelini; Faustino Torrico; Carlos Robello; Patricio Diosque; Omar Triana Chavez; Christine Aznar; Graciela Russomando; Philippe Büscher; Azzedine Assal; Felipe Guhl; Sergio Sosa Estani; Alexandre DaSilva; Constan?a Britto; Alejandro Luquetti; Janis Ladzins;
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1. Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients [J] . Carlos Ramirez Juan, Ines Cura Carolina, da Cruz Moreira Otacilio, The Journal of molecular diagnostics: JMD . 2015,第5期

机译：定量实时PCR方法定量分析南美锥虫病患者血样中锥虫锥虫DNA的分析验证
2. A simple protocol for the physical cleavage of Trypanosoma cruzi kinetoplast DNA present in blood samples and its use in polymerase chain reaction (PCR)-based diagnosis of chronic Chagas disease [J] . C. Britto, C. M. Morel, M. A. Cardoso, Memórias do Instituto Oswaldo Cruz . 1993,第1期

机译：血样中锥虫锥虫动植物体DNA的物理裂解的简单协议及其在基于聚合酶链反应（PCR）的慢性南美锥虫病诊断中的应用
3. Detection of Trypanosoma cruzi DNA in blood by PCR is associated with Chagas cardiomyopathy and disease severity [J] . Sabino E. C., Ribeiro A. L., Lee T. H., European journal of heart failure: journal of the Working Group on Heart Failure of the European Society of Cardiology . 2015,第4期

机译：PCR检测血液中克氏锥虫DNA与南美锥虫性心肌病和疾病严重程度有关
4. A Multiagent-based Simulation of the Infection of the Macrophage by Trypanosoma Cruzi in the Acute Phase of Chagas' Disease: Influence of the Initial Inoculum and Protozoan Escape Factor [C] . Willian Cordeiro Farago, Alcione de Paiva Oliveira, Rodrigo Siqueira-Batista, International Conference on Enterprise Information Systems . 2016

机译：基于多噬细胞急性疾病急性期巨噬细胞感染的多元模拟：初始接种物和原生动物逃生因子的影响
5. Isolation and Characterization of α-Gal-Containing Extracellular Vesicles (EVs) from Three Major Genotypes of Trypanosoma cruzi: Potential Biomarkers of Chagas Disease [D] . Hosseini, Nasim Karimi. 2020

机译：从胰蛋白酶瘤Cruzi三大基因分型的α-加仑细胞外囊泡（EVS）的分离与表征：抑菌病的潜在生物标志物
6. International Study to Evaluate PCR Methods for Detection of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients [O] . Alejandro G. Schijman, Margarita Bisio, Liliana Orellana, 2011

机译：评价南美锥虫病患者血液样本中克氏锥虫DNA检测PCR方法的国际研究
7. International Study to Evaluate PCR Methods for Detection of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients [O] . Ro G. Schijman, Margarita Bisio, Liliana Orellana, 2015

机译：国际研究评估鉴别pCR方法检测南美锥虫病患者血液中的克氏锥虫DNa

International Study to Evaluate PCR Methods for Detection of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients

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