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首页> 外文期刊>Proteome science >Differential abundance analysis of mesocarp protein from high- and low-yielding oil palms associates non-oil biosynthetic enzymes to lipid biosynthesis
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Differential abundance analysis of mesocarp protein from high- and low-yielding oil palms associates non-oil biosynthetic enzymes to lipid biosynthesis

机译：高产和低产油棕中果皮蛋白质的差异丰度分析将非油生物合成酶与脂质生物合成相关联

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Background The oil palm Elaeis guineensis Jacq. which produces the highest yield per unit land area of the oil crops is the most important commercial oil crop in South East Asia. The fleshy mesocarp of oil palm fruit, where oil is mostly derived from, contains up to 90?% dry weight of oil (one of the most concentrated in plant tissues). Hence, there is attention given to gain insights into the processes of oil deposition in this oil rich tissue. For that purpose, two-dimensional differential gel electrophoresis (DIGE) coupled with western assays, were used here to analyze differential protein levels in genetically-related high-and low-yielding oil palm mesocarps. Results From the DIGE comparative analysis in combination with western analysis, 41 unique differentially accumulated proteins were discovered. Functional categorization of these proteins placed them in the metabolisms of lipid, carbohydrate, amino acids, energy, structural proteins, as well as in other functions. In particular, higher abundance of fructose-1,6-biphosphate aldolase combined with reduced level of triosephosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase may be indicative of important flux balance changes in glycolysis, while amino acid metabolism also appeared to be closely linked with oil yield. Conclusions Forty-one proteins in several important biological pathways were identified as exhibiting differential in abundance at critical oil production stages. These confirm that oil yield is a complex trait involving the regulation of genes in multiple biological pathways. The results also provide insights into key control points of lipid biosynthesis in oil palm and can assist in the development of genetic markers for use in oil palm breeding programmes.
机译：背景油棕Elaeis guineensis Jacq。该地区单产最高的油料作物是东南亚最重要的商业油料作物。油棕果实的肉质中果皮主要来自油，含有高达90％的干重油（植物组织中最浓的一种）。因此，需要注意以了解这种富含油的组织中的油沉积过程。为此，这里使用了二维差异凝胶电泳（DIGE）和Western分析，来分析遗传相关的高产和低产油棕中果皮中的差异蛋白质水平。结果通过DIGE比较分析与Western分析相结合，发现了41种独特的差异积累蛋白。这些蛋白质的功能分类将它们置于脂质，碳水化合物，氨基酸，能量，结构蛋白以及其他功能的代谢中。特别是，较高的果糖-1,6-二磷酸醛缩醛酶结合降低的磷酸三糖异构酶和甘油醛-3-磷酸脱氢酶水平可能表明糖酵解中重要的通量平衡变化，而氨基酸代谢也似乎与糖酵解密切相关。石油产量。结论在几个重要的生物途径中，有41种蛋白质被确定在关键采油阶段表现出丰富的差异。这些证实了石油产量是一个复杂的性状，涉及多种生物途径中基因的调控。该结果还提供了对油棕中脂质生物合成关键控制点的见识，并可以帮助开发用于油棕育种计划的遗传标记。

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