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首页> 外文期刊>Proteome science >Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation
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Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation

机译：伽玛射线辐照后最早阶段沙漠沙门氏菌的主要可溶性蛋白质组变化

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Background Deinococcus deserti VCD115 has been isolated from Sahara surface sand. This radiotolerant bacterium represents an experimental model of choice to understand adaptation to harsh conditions encountered in hot arid deserts. We analysed the soluble proteome dynamics in this environmentally relevant model after exposure to 3 kGy gamma radiation, a non-lethal dose that generates massive DNA damages. For this, cells were harvested at different time lapses after irradiation and their soluble proteome contents have been analysed by 2-DE and mass spectrometry. Results In the first stage of the time course we observed accumulation of DNA damage response protein DdrB (that shows the highest fold change ~11), SSB, and two different RecA proteins (RecAP and RecAC). Induction of DNA repair protein PprA, DNA damage response protein DdrD and the two gyrase subunits (GyrA and GyrB) was also detected. A response regulator of the SarP family, a type II site-specific deoxyribonuclease and a putative N-acetyltransferase are three new proteins found to be induced. In a more delayed stage, we observed accumulation of several proteins related to central metabolism and protein turn-over, as well as helicase UvrD and novel forms of both gyrase subunits differing in terms of isoelectric point and molecular weight. Conclusions Post-translational modifications of GyrA (N-terminal methionine removal and acetylation) have been evidenced and their significance discussed. We found that the Deide_02842 restriction enzyme, which is specifically found in D. deserti, is a new potential member of the radiation/desiccation response regulon, highlighting the specificities of D. deserti compared to the D. radiodurans model.
机译：背景技术从撒哈拉沙漠的地表沙中分离出Deinococcus deserti VCD115。这种耐辐射细菌代表了一种选择的实验模型，以了解对干旱热沙漠中遇到的恶劣条件的适应性。我们在暴露于3 kGyγ辐射（产生大量DNA损伤的非致命剂量）后，分析了该环境相关模型中的可溶性蛋白质组动力学。为此，在照射后的不同时间收获细胞，并通过2-DE和质谱法分析了它们的可溶性蛋白质组含量。结果在时间过程的第一阶段，我们观察到了DNA损伤应答蛋白DdrB（显示最大的倍数变化〜11），SSB和两种不同的RecA蛋白（RecAP和RecAC）的积累。还检测到了DNA修复蛋白PprA，DNA损伤应答蛋白DdrD和两个促旋酶亚基（GyrA和GyrB）的诱导。 SarP家族的应答调节剂，II型位点特异性脱氧核糖核酸酶和推定的N-乙酰基转移酶是发现的三种新蛋白。在一个更延迟的阶段，我们观察到几种与中枢代谢和蛋白质转换有关的蛋白质的积累，以及解旋酶UvrD和两种回旋酶亚基的新型形式，其等电点和分子量不同。结论已经证明了GyrA的翻译后修饰（N末端甲硫氨酸的去除和乙酰化），并讨论了它们的重要性。我们发现Deide_02842限制酶（特别是在D. deserti中发现）是辐射/干燥反应调节剂的新潜在成员，与D. radiodurans模型相比，突出了D. deserti的特异性。

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