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首页> 外文期刊>Pure and Applied Biology >Chemical composition, antimicrobial and antileishmanial activity of essential oil of Juniperus excelsa M.Bieb. from Ziarat, Balochistan
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Chemical composition, antimicrobial and antileishmanial activity of essential oil of Juniperus excelsa M.Bieb. from Ziarat, Balochistan

机译：杜松的精油的化学成分，抗菌和抗衰老活性。来自Balochistan的Ziarat

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摘要

Essential oils isolated from leaves, barriers and from their mixture of Juniper ( Juniperus excelsa M.Bieb ) growing in Ziarat district. Essential oils were obtained by Clevenger-type apparatus, and components identified by gas chromatography mass spectroscopy (GC-MS). A total 27 components were identified which were inconsistent in their composition in the essential oils of J.excelsa , where five major variable components were: limonene (15.5-28.59%), followed by α-pinene (5.01-14.04%), cedrol (4.68-14.5%), δ-elemen (6.9-13.01%) and δ-cadinene (5.2-12.06%). Essential oils were evaluated for their in vitro antimicrobial activity against five bacterial species by disk diffusion method. J. excelsa essential oils showed antibacterial activity against tested organisms whilst the highest inhibition zone was observed for E. coli . Antileishmanial activity of essential oils isolated from juniper leaves, barriers and their mixture were also determined by using different concentration from (0.01ng/ml to 100 μg/ml). The IC50 value of all three essential oils were 0.0065 μg/ml, 0.0088 μg/ml and 0.0093 μg/ml respectively and demonstrated almost 100 times more antileishmanial activity in comparison to the standard Amphotericin B.
机译：从叶子，屏障和在齐亚拉特地区生长的杜松（Juniperus excelsa M.Bieb）混合物中分离出的香精油。通过Clevenger型仪器获得精油，并通过气相色谱质谱法（GC-MS）鉴定组分。共有27种成分在J.excelsa精油中组成不一致，其中五个主要可变成分为：柠檬烯（15.5-28.59％），其次是α-pine烯（5.01-14.04％），雪松酚（ 4.68-14.5％），δ-元素（6.9-13.01％）和δ-cad烯（5.2-12.06％）。通过圆盘扩散法评估了香精油对五种细菌的体外抗菌活性。 J. excelsa精油对被测生物具有抗菌活性，而对大肠杆菌的抑制作用最高。还使用从（0.01ng / ml到100μg/ ml）不同的浓度测定了从杜松叶，屏障及其混合物中分离出的香精油的抗leishmanial活性。这三种精油的IC 50 值分别为0.0065μg/ ml，0.0088μg/ ml和0.0093μg/ ml，与标准的两性霉素B相比，其抗细菌活性提高了近100倍。

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