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Validation of methodology for the chemical characterization of sugar cane bagasse

机译：甘蔗渣的化学表征方法的验证

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In this work, a methodology for the characterization of sugar cane bagasse was validated. Bagasse pre-treated with steam in a 5000 L reactor at a pressure of 15.3 kgf/cm2, during 7 min, was used to test the methodology. The methodology consisted of the hydrolysis of the material with H2SO4 at 72% v/v, for the quantification of carbohydrates, organic acid, furfural and hydroxymethylfurfural by HPLC; insoluble lignin and ash by gravimetry; and soluble lignin by spectrophotometry. Linearity, repeatability, reproducibility and accuracy of the results obtained in two Research Laboratories were determined, and were considered to be suitable for the validation of the methodology.
机译：在这项工作中，已验证了表征甘蔗渣的方法。使用甘蔗渣在5000 L反应器中以15.3 kgf / cm2的压力在7分钟内用蒸汽进行了预处理，以测试该方法。该方法包括用72％v / v的H2SO4水解材料，以通过HPLC定量碳水化合物，有机酸，糠醛和羟甲基糠醛。重量法测定不溶性木质素和灰分;分光光度法测定可溶性木质素。确定了在两个研究实验室中获得的结果的线性，可重复性，可重复性和准确性，并认为它们适合于该方法的验证。

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