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Immobilized metal-ion affinity chromatography (IMAC) of biomolecules: fundamental aspects and technological applications

机译:固定化生物分子的金属离子亲和层析(IMAC):基本方面和技术应用

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Immobilized Metal Ion Affinity Cromatography - IMAC - is a group-specific based adsorption applied to the purification and structure-function studies of proteins and nucleic acids. The adsorption is based on coordination between a metal ion chelated on the surface of a solid matrix and electron donor groups at the surface of the biomolecule. IMAC is a highly selective, low cost, and easily scaled-up technique being used in research and commercial operations. A separation process can be designed for a specific molecule by just selecting an appropriate metal ion, chelating agent, and operational conditions such as pH, ionic strength, and buffer type.
机译:固定化金属离子亲和层析法-IMAC-是一种基于组特异性的吸附法,用于蛋白质和核酸的纯化和结构功能研究。吸附基于螯合在固体基质表面的金属离子与生物分子表面的电子给体基团之间的配位。 IMAC是一种高度选择性,低成本且易于扩展的技术,用于研究和商业运营。只需选择合适的金属离子,螯合剂和操作条件(例如pH值,离子强度和缓冲液类型),即可针对特定分子设计分离过程。

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