...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Ukrainian Biochemical Journal >Development and characterization of highly informative ELISA for the detection of IgG and IgA antibodies to Сhlamydia trachomatis
【24h】

Development and characterization of highly informative ELISA for the detection of IgG and IgA antibodies to Сhlamydia trachomatis

机译：用于沙眼衣原体的IgG和IgA抗体检测的高信息量ELISA的开发和表征

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The goal of this work was developing of highly informative an enzyme-linked immunosorbent assay (ELISA) for the detection of IgG and IgA antibodies against to Chlamydia trachomatis , as well as comparative characterization of developed assay using standardized control materials. The study was conducted using: monoclonal antibodies (McAbs) to human IgA and IgG; recombinant Ch. trachoma tis proteins – Pgp3, major outer membrane protein (MOMP); two panels of characterized sera and four reference ELISA kits. The study of immunochemical activity of peroxidase conjugates of McAbs was performed in comparison with conjugates of commercial analogues: anti-IgG McAb 2A11 and anti-IgA McAb AD3. About half of the conjugates from the received McAbs panel were more active compared to the reference antibody conjugates. It was quite justified to use the conjugates of antibodies that interact with different antigenic determinants. When IgG antibodies were detected to MOMP, it was justified 1.14-1.56 times more; when IgA antibodies were detected to MOMP, it was justified 1.16-1.37 times more. ELISA for detecting IgG/IgA antibodies to MOMP and Pgp3 of Ch. trachomatis were evaluated using appropriately described serum panels OCO-42-28-313-00 and OCO-42-28-314-00. Comparative studies of the developed ELISA for the detection of IgG and IgA antibodies to the MOMP and Pgp3 of Ch. trachomatis showed their prominent advantage over the commercial analogues, which more clearly demonstrates the difference in the ratio of average values of optical density of positive and negative samples of the described panel of sera: this indicator for commercial kits was 1.36-3.59 times less.
机译：这项工作的目标是开发一种高信息量的酶联免疫吸附测定（ELISA），用于检测抗沙眼衣原体的IgG和IgA抗体，以及使用标准化对照材料对开发的测定进行比较表征。该研究使用以下方法进行：针对人IgA和IgG的单克隆抗体（McAb）;重组Ch。沙眼tis蛋白– Pgp3，主要外膜蛋白（MOMP）;两份特征性血清和四份参考ELISA试剂盒。与商业类似物（抗IgG McAb 2A11和抗IgA McAb AD3）的结合物相比，对McAbs过氧化物酶结合物的免疫化学活性进行了研究。与参考抗体结合物相比，来自所接收的McAbs组的结合物的约一半更具活性。使用与不同抗原决定簇相互作用的抗体的缀合物是完全合理的。当检测到针对MOMP的IgG抗体时，其合理性要高1.14-1.56倍;当检测到针对MOMP的IgA抗体时，其合理性是1.16-1.37倍。 ELISA用于检测Ch。的MOMP和Pgp3的IgG / IgA抗体。使用适当描述的血清样本OCO-42-28-313-00和OCO-42-28-314-00对沙眼进行评估。已开发的用于检测Ch.MOMP和Pgp3的IgG和IgA抗体的ELISA的比较研究。沙眼病菌显示出其优于市售类似物的显着优势，这更清楚地证明了所述血清组的阳性和阴性样品的光密度平均值之比的差异：用于商业试剂盒的该指标少了1.36-3.59倍。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号