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首页> 外文期刊>Ukrainian Biochemical Journal >IRE1 knockdown modifies hypoxic regulation of cathepsins and LONP1 genes expression in U87 glioma cells
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IRE1 knockdown modifies hypoxic regulation of cathepsins and LONP1 genes expression in U87 glioma cells

机译：IRE1敲低修改U87胶质瘤细胞中组织蛋白酶和LONP1基因表达的低氧调节

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We have studied the effect of hypoxia on the expression of genes encoding for LONP1/PRSS15 and cathepsins in U87 glioma cells in relation to inhibition of inositol requiring enzyme-1 (IRE1). It was shown that hypoxia up-regulated the expression of CTSA , CTSB , CTSD , CTSF , CTSK , and LONP1 genes and down-regulated the expression of CTSC , CTSL , CTSO , and CTSS genes in control glioma cells (transfected by empty vector). Inhibition of ?RE1 signaling enzyme function in these glioma cells modified effect of hypoxia on the expression of most studied genes: eliminated the effect of hypoxia on CTSA , and LONP1 genes, introduced reverse changes on CTSD and CTSS genes, reduced – on CTSF and CTSK genes, and enhanced – on CTSB and CTSL genes. Therefore, hypoxia affected the expression level of most studied genes in relation to the functional activity of IRE1 enzyme, a central mediator of endoplasmic reticulum stress, which control cell proliferation and tumor growth.
机译：我们已经研究了缺氧对U87胶质瘤细胞中编码LONP1 / PRSS15和组织蛋白酶的基因表达的影响，这与抑制需要1酶的肌醇有关。结果表明，缺氧可上调胶质瘤细胞（空载体转染）中CTSA，CTSB，CTSD，CTSF，CTSK和LONP1基因的表达，并下调CTSC，CTSL，CTSO和CTSS基因的表达。 。在这些神经胶质瘤细胞中抑制？RE1信号酶功能改变了缺氧对大多数研究基因表达的影响：消除了缺氧对CTSA和LONP1基因的影响，对CTSD和CTSS基因引入了反向变化，对CTSF和CTSK降低了基因，并且在CTSB和CTSL基因上得到了增强。因此，缺氧影响与IRE1酶的功能活性有关的大多数研究基因的表达水平，IRE1酶是控制细胞增殖和肿瘤生长的内质网应激的中心介质。

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