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首页> 外文期刊>Ukrainian Biochemical Journal >Different mice inbred strains humoral immune response against human prostate-specific antigen
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Different mice inbred strains humoral immune response against human prostate-specific antigen

机译：不同小鼠自交系对人前列腺特异性抗原的体液免疫反应

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The aim of the study was an investigation of humoral immune response to prostate-specific antigen (PSA) of different mice inbred strains for further development of recommendations for appropriate immunization schemes for monoclonal antibodies (McAbs) obtaining. The study was conducted using: Balb/c and NZB mice; PSA from human sperm (as immunogen). In case of booster immunization immunogen was previously diluted to the desired concentration (10 or 30 μg per 100 μl) in saline, and injected in the tail vein or intraperitoneally. In case of other immunizations, we prepared emulsion solution of immunogen with adjuvant to final concentration of 10 or 30 μg per 100 μl: PSA was dissolved in saline, the same volume of adjuvant was added, and mixture was thoroughly mixed to form a stable emulsion. When subcutaneous administration, total dose of 100 μl was divided into two equal parts and injected in the hind paw of mice. Intraperitoneal immunization was performed by single administration of 100 μl of emulsion. The level of specific antibodies was determined by titration of blood sera from animals in indirect ELISA. Series of experiments were conducted to determine the level of humoral response of mice of different inbred strains (Balb/c and NZB) for multi-stage immunization with different duration with different amounts of PSA (10 and 30 μg), with different immunoglobulin administration (intraperitoneal and subcutaneously), and with different adjuvants (Freund’s complete and incomplete adjuvants, FCA/FIA). Final booster immunization at a dose of 30 μg was performed either in the same manner as the previous administration of the immunogen, or intravenously in the physiological saline solution. Dependencies of the humoral immune response of Balb/c and NZB mice against PSA on the route of administration of immunogen, the dose and duration of immunization were established. It was shown that intraperitoneal administration provided formation of higher titers of specific antibodies in case of both mice strains. Balb/c mice lines more rapidly responded to PSA, than an NZB mice (for all investigated schemes immunization). It was shown that the most effective immunization scheme was three times intraperitoneal administration with 10 μg of PSA for 8 weeks (the first immunization with FCA, and the rest – with FIA) and booster immunogen intravenous administration in saline solution.
机译：该研究的目的是研究对不同小鼠近交系对前列腺特异性抗原（PSA）的体液免疫反应，以进一步开发针对获得单克隆抗体（McAbs）的适当免疫方案的建议。该研究使用以下动物进行：Balb / c和NZB小鼠;来自人精子的PSA（作为免疫原）。在加强免疫的情况下，预先将免疫原在盐水中稀释至所需浓度（每100μl10或30μg），然后注入尾静脉或腹膜内。在进行其他免疫的情况下，我们制备了含佐剂的免疫原乳剂溶液，其终浓度为每100μl10或30μg：将PSA溶解在盐水中，加入相同体积的佐剂，并将混合物充分混合以形成稳定的乳剂。皮下给药时，将100μl的总剂量分成两个相等的部分，并注射到小鼠的后爪中。腹膜内免疫是通过单次施用100μl乳液进行的。特异性抗体的水平是通过间接ELISA法滴定动物的血液血清来确定的。进行一系列实验以确定不同自交系（Balb / c和NZB）的小鼠的体液反应水平，以不同的持续时间，不同的PSA量（10和30μg），不同的免疫球蛋白给药进行多阶段免疫（腹膜内和皮下注射），并使用不同的佐剂（弗氏完全和不完全佐剂，FCA / FIA）。以与先前给予免疫原相同的方式，或在生理盐水溶液中静脉内注射，以30μg的剂量进行最终的加强免疫。建立了Balb / c和NZB小鼠针对PSA的体液免疫应答对免疫原的给药途径，免疫剂量和持续时间的依赖性。结果表明，在两种小鼠品系的情况下，腹膜内给药都能形成更高滴度的特异性抗体。与NZB小鼠相比，Balb / c小鼠品系对PSA的反应更快（对于所有研究的免疫方案）。结果表明，最有效的免疫方案是腹腔内给予10μgPSA 3次，共8周（第一次使用FCA进行免疫，其余使用FIA进行免疫），并在盐溶液中静脉内加强免疫原。

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