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首页> 外文期刊>Polymers >2-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem Cells
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2-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem Cells

机译：甲基丙烯酸2-（二甲氨基）乙酯/甲基丙烯酸2-（羟乙氧基）酯/α-磷酸三钙晶状体，人小梁骨衍生细胞和间充质干细胞生物学反应的制备和评价

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The aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype.
机译：这项工作的目的是评估冷冻凝胶在组织工程中用作支架的潜力。制备了基于α-磷酸三钙增强的PDMAEMA（聚甲基丙烯酸二甲酯）/ PHEMA（聚甲基丙烯酸羟乙酯）系统的支架，并制备了人小梁骨衍生细胞（HTB）和骨髓衍生间充质干细胞（BM） -MSCs）。研究了诸如孔隙度，孔形状，交联之间的分子量和筛孔尺寸等几个特征。已经评估了最适合细胞增殖的PDMAEMA / PHEMA比，并评估了成骨生化标记物的生存力，粘附力，增殖和表达。 PDMAEMA / PHEMA比率会影响支架的孔隙率。对于PHEMA中的较大含量，已获得53％±5.7％的值;对于PDMAEMA中的较大含量，已获得75％±5.5％的值。聚合物比率还改变了孔的形状。 PDMAEMA中内容的增加也会导致网络网格大小的增加。每种组合物都是无细胞毒性的，接种的细胞对于BM-MSC和HTB都保持活力。因此，基于结构分析，PDMAEMA中含量较高的标本似乎为用作组织工程支架提供了更好的结构环境。将α-磷酸三钙掺入组合物中似乎有利于成骨表型的表达。

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