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首页> 外文期刊>The Internet Journal of Pharmacology >Effect Of Aegle Marmelos Correa On Blood Glucose Level In Normal And Alloxan- Induced Diabetic Albino Rats
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Effect Of Aegle Marmelos Correa On Blood Glucose Level In Normal And Alloxan- Induced Diabetic Albino Rats

机译：Aegle Marmelos Correa对正常和四氧嘧啶诱导的糖尿病白化大鼠血糖水平的影响

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The aim of the present study is to evaluate the action extract of Aegle marmelos correa on blood glucose level in normal and alloxan-induced diabetic albino rats. The aqueous extract (100mg/kg/d) was administered orally for four weeks to alloxan-induced diabetic rats. Blood glucose was estimated every week for four consecutive weeks. For evaluation of mechanism of action of test drug, glycogen estimation was done in liver, heart and skeletal muscle and effect on adrenaline-induced hyperglycemia was seen. The test drug significantly (p<0.01) reduced the rise in blood glucose induced by alloxan. The test drug produced significant (p<0.01) increase in liver glycogen and also significantly (p<0.01) reduced adrenaline-induced hyperglycaemia. No significant lowering of normal blood glucose was also found. Thus, the fruit of Aegle marmelos has significant antidiabetic activity. Introduction Diabetes mellitus (DM) refers to a group of common metabolic disorders that share the phenotype of hyperglycemia1. A changing lifestyle in developing countries like India has enormously increased the statistical figures of diabetes mellitus. A survey depicts that 4% of adults in India suffered from DM in year 20000 and it is expected to increase by 6% by the year 2025.2 Aegle marmelos correa is a tree belonging to the family Rutaceae, occuring throughout India, Srilanka, Pakistan, Bangladesh, Myanmar, Thailand and most Southeastern Asian countries. Aegle marmelos correa commonly known as Bael fruit is mildly astringent and used to cure dysentry, diarrhea,3 hepatitis, tuberculosis, dyspepsia and good for heart and brain.4 Phytochemical analysis of bael fruit has revealed the presence of alkaloids, coumarin, marmin, imperatorin, aegelin, tannins .5 The powdered bael fruit is used by the local people of Assam in the treatment of diabetes. Keeping this in view, the present study was aimed at evaluating the effect of Aegle marmelos correa on blood glucose level in normal and alloxan-induced diabetic albino rats with stress on evaluation of probable mechanism of antidiabetic action. Materials and Methods Plant material and extraction The fruit pulp of Aegle marmelos were collected from the local market in Dibrugarh in the months of June to August. The plant material was authenticated by Dr S.S.Dhawan, Professor of food and nutrition department, HAU, Hisar, Haryana.The fruit pulp of Aegle marmelos were manually separated from the outer seed shell, air dried, powdered (1600 g) and aqueous extracts were prepared using sufficient water by percolation method followed by steam evaporation. A final yield of 165 g of the extract was obtained.6 AnimalsHealthy adult Wistar albino rats (Rattus norvegicus) weighing 200—250 grams each were used for the study. All the animals were taken care of under ethical consideration as per the guidelines of the CPCSEA with due approval from the Institutional Animal Ethical Committee (Registration no: 634/02/a/CPCSEA; dated 19/5/2002). Chemicals usedCrude powder of glibenclamide was obtained from Sun Pharma Ltd., mumbai while alloxan monohydrate was purchased from Sigma Aldrich India, Bangalore. The glucose kit for blood glucose estimation was obtained from Sigma Diagnostic (India) Pvt. Ltd., Baroda.Experimental design for antidiabetic study A total of thirty animals were equally divided into five groups with six animals in each group: Group–A : Normal Control. Received normal saline, 2 ml/kg/d. Group- B: Normal Test. Received normal saline, 2 ml/kg/d. Group–C: Diabetic Control. Received normal saline, 2 ml/kg/d. Group–D: Diabetic Test. Received aqueous extract of Aegle marmelos (AEAM), 100 mg/kg/d. Group–E: Diabetic Standard. Received glibenclamide, 2.5 mg/kg/d.The above drugs were administered orally, once daily, for four weeks. Induction of diabetesLeaving aside six rats for Normal Control Group, 24 rats were induced diabetes by a single intraperitoneal injection of alloxan monohydrate in the dose of 150 mg/kg body weight. Th
机译：本研究的目的是评估在健康的和四氧嘧啶诱导的糖尿病白化病大鼠中，金银花提取物对血糖水平的作用。将水提取物（100mg / kg / d）经口服给予四氧嘧啶诱导的糖尿病大鼠四周。每周连续四个星期估计血糖。为了评估受试药物的作用机理，在肝，心脏和骨骼肌中进行了糖原估计，并观察到了对肾上腺素诱导的高血糖的影响。受试药物显着（p <0.01）降低了四氧嘧啶引起的血糖升高。受试药物可使肝糖原显着增加（p <0.01），并降低肾上腺素诱导的高血糖（p <0.01）。还没有发现正常血糖的显着降低。因此，Aegle marmelos的果实具有显着的抗糖尿病活性。简介糖尿病（DM）是指一组具有高血糖表型的常见代谢性疾病。在印度等发展中国家，生活方式的改变极大地增加了糖尿病的统计数字。一项调查显示，到20000年，印度有4％的成年人患有DM，到2025.2年，预计将增加6％。Aegle marmelos correa是芸香科的一棵树，遍布印度，斯里兰卡，巴基斯坦，孟加拉国，缅甸，泰国和大多数东南亚国家/地区。 Aegle marmelos correa通常被称为Bael水果，略带涩味，可用于治疗痢疾，腹泻，3肝炎，肺结核，消化不良，对心脏和大脑有益。4对Bael水果的植物化学分析表明，存在生物碱，香豆素，marmin，imperatorin ，aegelin，单宁.5粉末状的贝尔水果被阿萨姆邦人用于治疗糖尿病。鉴于此，本研究旨在评估Aegle marmelos correa对正常和四氧嘧啶诱导的糖尿病白化病大鼠血糖水平的影响，以评估其可能的抗糖尿病作用机制。材料和方法植物材料和提取6月至8月的几个月，从Dibrugarh的当地市场收集了Aegle marmelos的果肉。植物材料由哈里亚纳邦希萨尔市HAU食品和营养学系教授SSDhawan博士认证，将Aegle marmelos的果肉手动从种子壳中分离出来，风干，粉化（1600 g），并用水提取通过渗滤方法使用足够的水，然后进行蒸汽蒸发来制备。最终提取物的产量为165g。6动物每只体重200-250克的健康成年Wistar白化病大鼠（Rattus norvegicus）用于研究。在得到机构动物伦理委员会的适当批准（登记号：634/02 / a / CPCSEA; 2002年5月19日）的情况下，根据CPCSEA的准则，对所有动物进行了道德考量。所用化学药品格列本脲粗粉购自孟买的Sun Pharma Ltd.，一水合四氧嘧啶购自班加罗尔的Sigma Aldrich India。用于血糖估计的葡萄糖试剂盒可从Sigma Diagnostic（India）Pvt获得。 Ltd.，Baroda。抗糖尿病研究的实验设计将总共30只动物平均分为五组，每组六只动物：A组：正常对照组。接受生理盐水2 ml / kg / d。 B组：正常测试。接受生理盐水2 ml / kg / d。 C组：糖尿病控制。接受生理盐水2 ml / kg / d。 D组：糖尿病检查。收到的马格洛斯岛水提取物（AEAM），100 mg / kg / d。 E组：糖尿病标准。接受格列本脲2.5 mg / kg / d。以上药物每天口服一次，持续4周。糖尿病的诱导除了正常对照组的六只大鼠外，通过腹膜内一次注射剂量为150 mg / kg体重的四氧嘧啶一水合物来诱发24只糖尿病。钍

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