Anti-diabetic activity of stem bark of Berberis aristata D.C. in alloxan induced diabetic rats

Bhupesh Chander Semwal; Kamal Shah; Nagendra Singh Chauhan; Rohit Badhe; Kalyani Divakar

摘要

Object: To evaluate antidiabetic activity of stem bark of Berberis aristata D.C. (Berberdiaceae) in alloxan induced diabetic rats. Materials and Method: Male Wistar albino rats (150–250 g) were taken and study for glucose tolerance test and alloxan induced diabetic rats. The ethanolic extract of B. aristata were selected for antidiabetic activity. Blood glucose levels were estimated in all groups on 1st, 4th, 7th and 15th day of the treatment with B. aristata. The biochemical parameters and body weight were estimated all treated groups and compared against diabetic control group. Results: The ethanolic extract of stem bark of B. aristata showed a significant hyperglycemic effect in alloxan induced diabetic rats. It reduces blood glucose level 60.4% and & 75.46 % at the dose of 25 mg/kg and 50 mg/kg in diabetic rats. B. aristata has a significant antidiabetic activity in glucose tolerance test Conclusion: The results justify the traditional use of bark in the treatment of diabetes. Introduction Diabetes mellitus is one of the common metabolic disorders with micro and macro vascular complications that results in significant morbidity and mortality. It is considered as one of the five leading causes of death in the world 1,2 . About 150 million or 1.3% people are suffering from diabetes world wide which is almost five times more than the estimates ten years ago and this may double by the year 2030 3 . Diabetes was discovered as early as 700-200 BC; until the time insulin was invented, this disorder was managed principally by the traditional practices by using medicinal plants 4 There are numerous traditional medicinal plants reported to have hypoglycemic properties such as Allium sativum (Garlic), Azadirachta indica (Neem), Vinca rosea (Nayantara), Trigonalla foenum (Fenugreek), Momordica charantia (Bitter ground), Ocimum santum (Tulsi) many of these are less effective in lowering glucose levels in severe diabetes. Berberis aristata D.C. (Berberidaceae) is an edible plant commonly used in ayurvedic system of medicine as in diarrhoea, hypoglycemic, anticancer, gastro-irritant, antipyretic, hypotensive, CNS depressant and diaphoretic 5,6,7 . However no scientific study on anti-diabetic activity of this plant has been reported. The present investigation was undertaken to study the anti-diabetic activity of stem bark of B. aristata in alloxan induced diabetic rats. Materials and methods Plant materialPlant material used in this study consisted of the stem bark of B. aristata, collected from the local area of Rishikesh, Uttranchal. The plant was authenticated by Dr. H. J. Chaudhary, Director of Botanical Survey of India, Govt. of India, Dehradun Bsi/Nc/6(4)/06-07. The stem bark was dried in shade at room temperature. Dried stem bark was coarsely powdered by using grinder, and latter was packed into soxhlet column and that was defatted with petroleum ether then extracted with ethanolic and dried in vacuum. The presence of alkaloids were confirmed by qualitative examination. AnimalsMale Wistar albino rats (150–250 g) housed in a spacious cage for ten days after obtaining approval from ‘Institutes Ethical Committee' 997/c/06/CPCSEA. During the experiments rats were feeded with standard pellet diet. After randomization into various groups, the rats were acclimatized for 2–3 days in environment before initiation of experiment.Glucose tolerance testRats were divided into four groups containing six animals in each group. All animals fasted before treatment. Group I was kept as vehicle control which received 5% Tween 80 p.o., group II received glucose only, group III received ethanolic extract 25 mg/kg and group IV received only extract only in a vehicle respectively .The rats of group II and III were loaded with glucose (3 g/kg, p.o.) 30 min after drug administration. Blood samples were collected from puncturing the retro orbital sinus just prior to drug administration, and 30, 90, 150 min after loading glucose. Serum glucose level was measu

机译：目的：评价小Ber（Berberis aristata D.C。）（小ber科）的茎皮在四氧嘧啶诱导的糖尿病大鼠中的抗糖尿病活性。材料和方法：取雄性Wistar白化病大鼠（150–250 g），研究其葡萄糖耐量试验和四氧嘧啶诱导的糖尿病大鼠。选择硬脂酸杆菌的乙醇提取物具有抗糖尿病活性。在用牛油杆菌治疗的第1、4、7和15天，估计所有组的血糖水平。估计所有治疗组的生化参数和体重，并与糖尿病对照组进行比较。结果：棉铃虫茎皮乙醇提取物在四氧嘧啶致糖尿病大鼠中显示出明显的降血糖作用。在糖尿病大鼠中，在25 mg / kg和50 mg / kg的剂量下，它降低了60.4％和75.46％的血糖水平。结论：该结果证明了树皮在糖尿病治疗中的传统用途是合理的。简介糖尿病是常见的代谢疾病之一，具有微血管和大血管并发症，会导致较高的发病率和死亡率。它被认为是世界上五个主要死因之一1,2。全世界约有1.5亿或1.3％的人患有糖尿病，这比十年前的估计数高出将近五倍，到2030年可能翻一番3。糖尿病早在公元前700-200年就被发现。在发明胰岛素之前，主要通过传统方法使用药用植物来控制这种疾病4。据报道，许多具有降血糖特性的传统药用植物，如大蒜，大蒜，尼古拉，长春花（Nayantara）。），三角叶茴香（胡芦巴），苦瓜（苦瓜），桑皮（Tulsi），其中许多在降低严重糖尿病患者的血糖水平方面效果较差。小ber（Derberis aristata D.C.）（小er科）是一种可食用植物，通常用于腹泻，降血糖，抗癌，胃刺激性，退热，降压，中枢神经系统抑制药和发炎药的阿育吠陀系统中5,6,7。然而，尚未有关于该植物抗糖尿病活性的科学研究的报道。进行本研究以研究阿里斯塔德酵母茎皮在四氧嘧啶诱导的糖尿病大鼠中的抗糖尿病活性。材料和方法植物材料本研究中使用的植物材料由B. aristata的茎皮组成，该树皮取自Uttranchal的Rishikesh地区。该植物经印度政府植物调查局局长H. J. Chaudhary博士认证。印度，Dehradun Bsi / Nc / 6（4）/ 06-07。将茎皮在室温下阴凉处干燥。用研磨机将干燥的茎皮粗粉，然后将其装填到索氏萃取柱中，用石油醚脱脂，然后用乙醇萃取并真空干燥。通过定性检查确认了生物碱的存在。动物获得“ Institutes Ethical Committee” 997 / c / 06 / CPCSEA的批准后，将雄性Wistar白化病大鼠（150–250 g）在宽敞的笼子中饲养十天。在实验过程中，给大鼠饲喂标准的颗粒饲料。随机分为各组后，在实验开始前将大鼠在环境中适应2–3天。糖耐量测试大鼠分为四组，每组六只动物。治疗前所有动物禁食。将第I组作为媒介物对照组，其接受5％吐温80 po，第II组仅接受葡萄糖，第III组仅接受25 mg / kg的乙醇提取物，而第IV组仅在媒介物中仅接受提取物。药物给药后30分钟加载葡萄糖（3 g / kg，口服）。刚好在给药前和加载葡萄糖后30、90、150分钟从穿刺眶后窦收集血样。血清葡萄糖水平为标准

Anti-diabetic activity of stem bark of Berberis aristata D.C. in alloxan induced diabetic rats

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