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首页> 外文期刊>The Internet Journal of Pharmacology >Evaluation of anti-inflammatory activity of methanol extract of Barleria Cristata leaves by in vivo and in vitro methods
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Evaluation of anti-inflammatory activity of methanol extract of Barleria Cristata leaves by in vivo and in vitro methods

机译：体内和体外方法评价巴氏杆菌甲醇提取物的抗炎活性

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The methanol extract of Barleria Cristata leaves (BCM) was evaluated for anti-inflammatory activity using in vivo and in vitro methods. In the in vivo inflammation tests, BCM significantly inhibited edema produced by histamine and serotonin in rats, also reduces significantly acetic acid-induced vascular permeability in mice dose dependently. In the in vitro tests, the probable supporting mode by which BCM mediates its effects on inflammatory conditions was studied on red blood cells (RBC’s) exposed to hypotonic solution and thermally induced protein denaturation. BCM exhibited significant membrane-stabilizing property. Thermal induced protein denaturation was significantly inhibited by the extract. The effect was compared with the activity of indomethacin and cyproheptadine as reference standard against different types of inflammation. Results of the study revealed that BCM possesses significant anti-inflammatory activity. Source of financial support received This work was fully supported by grants from University Grants Commission, India. Introduction Medicinal plants are believed to be an important source of new chemical substances with potential therapeutic effects. The research into plants with alleged folkloric use as anti-inflammatory agents should therefore be viewed as a fruitful and logical research strategy in the search for new anti-inflammatory drugs[1]. Barleria Cristata Linn (family Acanthaceae) is a shrub found widely in subtropical Himalaya, Sikkim, Khasi Hills, central and southern India at height of 1,350 m. The chemical constituents of the plant have been identified as flavonoid type phenolic compounds especially apigenin, quercetin, quercetin-3-O-β-D-glucoside, naringenin and apigenin glucuronide[2]. Different parts of Barleria Cristata have been used traditionally for the treatment of variety of diseases including anaemia, toothache, cough and as a hypoglycemic agent. Roots and leaves were used to reduce swellings in inflammation [2]. However, there is no systematic scientific report published indicating utility of this plant material in the treatment of inflammation. Thus the presence of therapeutically active flavonoids as major constituents was the basis of selection and evaluation methanol extract of Barleria Cristata leaves for their anti-inflammatory activity. Materials And Methods Plant materialFresh leaves of the plant Barleria Cristata Linn were collected from Mumbai region, India. The plant material was taxonomically identified by Dr. Ganesh Iyer, Prof. in Botany, Ramnarain Ruia college, Mumbai, India. A voucher specimen (No. 9-1/08) has been preserved in our laboratory for future reference. The leaves were dried under shade and then powdered with a mechanical grinder and stored in airtight container. The dried powder material of the leaves was defatted with petroleum ether (60-80) and subsequently extracted with methanol in a soxhlet apparatus. The solvent was completely removed under reduced pressure and methanol extract of Barleria Cristata leaves (BCM) was obtained (yield 12.4%). Solution of BCM was prepared freshly in distilled water and used for the studies. Phytochemical screeningThe BCM extract was screened for the presence of various phytochemical constituents i.e. steroids, alkaloids, tannins, flavonoids, glycosides, etc by employing standard screening tests [3].Chemicals and drugsHistamine, Serotonin, Egg albumin and Evan’s blue were obtained from Himedia Lab [(Mumbai) India], Indomethacin [Recon, (Bangalore) India], Cyproheptadine [Fleming Laboratories Limited, (Hydrabad), India] and all other chemicals used were of analytical grade.AnimalsWistar albino rats of either sex weighing 180–200 g and Swiss albino mice of either sex weighing 18–22 g were used for animal studies. The animals were grouped in polyacrylic cages and maintained under standard laboratory conditions (temperature 25 ± 2 °C) and relative humidity (50±5%) with dark and light cycle (14/10 h). They were allowed free acc
机译：使用体内和体外方法评估了巴氏杆菌叶子（BCM）的甲醇提取物的抗炎活性。在体内炎症测试中，BCM显着抑制了大鼠中组胺和血清素产生的水肿，并且剂量依赖性地显着降低了乙酸诱导的小鼠血管通透性。在体外测试中，对暴露于低渗溶液和热诱导的蛋白质变性的红细胞（RBC）进行了研究，研究了BCM介导其对炎症状况的可能支持方式。 BCM表现出显着的膜稳定性能。提取物明显抑制了热诱导的蛋白质变性。将该效果与消炎痛和赛庚啶作为参考标准针对不同类型的炎症的活性进行了比较。研究结果表明，BCM具有明显的抗炎活性。收到的财政支持来源这项工作得到了印度大学资助委员会的资助的完全支持。简介药用植物被认为是具有潜在治疗作用的新化学物质的重要来源。因此，在被认为是民俗用途的抗炎药的植物研究应被视为寻找新的抗炎药的一项富有成果和合乎逻辑的研究策略[1]。 Barleria Cristata Linn（鼠李科）是一种灌木，广泛分布于印度中南部的亚热带喜马拉雅山，锡金，卡西山，印度中部和南部，高度为1,350 m。该植物的化学成分已确定为类黄酮类酚类化合物，尤其是芹菜素，槲皮素，槲皮素-3-O-β-D-葡萄糖苷，柚皮苷和芹菜素葡糖苷酸[2]。传统上，Barleria Cristata的不同部位已用于治疗各种疾病，包括贫血，牙痛，咳嗽和降糖药。根和叶用于减轻炎症肿胀[2]。然而，没有公开的系统科学报告表明该植物材料在炎症治疗中的实用性。因此，具有治疗活性的类黄酮作为主要成分的存在是选择和评估Barstatia Cristata叶子的甲醇提取物的抗炎活性的基础。材料和方法植物材料Barleria Cristata Linn的新鲜叶子采自印度孟买地区。该植物材料由印度孟买Ramnarain Ruia学院植物学教授Ganesh Iyer博士进行了分类鉴定。凭证样本（编号9-1 / 08）已保存在我们的实验室中，以备将来参考。叶片在阴凉处干燥，然后用机械研磨机粉化并储存在密闭容器中。叶子的干燥粉末材料用石油醚（60-80）脱脂，然后在索氏提取器中用甲醇萃取。在减压下将溶剂完全除去，并且获得了Barleria Cristata叶片（BCM）的甲醇提取物（收率12.4％）。 BCM溶液在蒸馏水中新鲜制备，并用于研究。植物化学筛选通过标准筛选试验[3]，对BCM提取物中的各种植物化学成分进行了筛选，包括类固醇，生物碱，单宁，类黄酮，糖苷等。化学药品Histamine，5-羟色胺，蛋清和Evan's blue购自Himedia Lab。 [（印度孟买），吲哚美辛[Recon，（印度班加罗尔）]，赛庚啶[Fleming Laboratories Limited，（印度海德拉巴德）]和所有其他化学药品均为分析纯。以及体重在18至22克之间的瑞士白化病小鼠进行了动物研究。将动物分组在聚丙烯笼中，并保持在标准实验室条件（温度25±2°C）和相对湿度（50±5％）下，进行黑暗和明亮的循环（14/10 h）。他们被允许免费使用

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