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首页> 外文期刊>The Internet Journal of Neurology >Brain Functional Localization Of Kidling Cats: Manganese Ion Enhances T1-Weighted MRI Study
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Brain Functional Localization Of Kidling Cats: Manganese Ion Enhances T1-Weighted MRI Study

机译：猫猫的脑功能定位：锰离子增强T1加权MRI研究

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Purpose: Our previous studies have demonstrated that calcium overloading is involved in the epileptogenesis. Mn2+ is handled in a manner similar to Ca2+ in many biological systems and can act as an excellent MRI contrast agent, so our present study was performed to determine the encephalic region correlated to epileptogenesis by Mn2+-fMRI and further to determine the correlation of epilepsy and calcium overloading. Methods The cats were divided into two groups, the first group was divided into group A and B, A group was PTZ experimental group, B group was normal saline (NS) controlling group, The first group was used to EEG examination, the adult male cats were combined anesthetized with ketamine and chlorderazin, with intramuscular injected of PTZ (55mg/kg), the ethological and EEG changes were observed. The second group was divided into group A and B, group A was used to Mn2+-fMRI of the acute epileptic seizures, group B was used to Mn2+-fMRI at the time of 24 hours after the acute epileptic seizures were induced, group C was control group NS. The adult male cats were combined anesthesia with ketamine and chlorderazin; two femoral veins were taken one for Mncl2 infusion, the other for mannitol infusion. The cats were injected with PTZ intramuscular, and the changes were observed under TI-Weighted MRI. Signal enhanced encephalic regions were dislodged to be done. Results: The ratio of kindling cats was 80% with intramuscular injection of PTZ, the EEG were paroxysmal with spike-slow waves of high amplitude, the etiology and EEG of control group were normal. When the cats showed generalized tonic-clonic convulsive seizures, cerebral cortex showed diffusely signal enhancement, the enhancement rate of frontal-parietal-occipital lobe was 34.6; the enhancement rate of temporal lobe was 22.9, and they had significant difference compared to the control groups. The Mn2+-fMRI at the time of 24 hours after epileptic seizures attacked also showed signal enhancement on frontal-parietal lobe. The neurons of enhanced encephalic regions show obvious degeneration and necrosis. Conclusion Cats can be succeeded to kindling by intramuscular PTZ (55mg/kg). Frontal-parietal lobe was the correlated encephalic regions of epilepsy, Mn2+-fMRI has important role on the localization and revealing pathogenesis of epileptic seizures. Introduction Felix et al [1] have demonstrated that enhancement of Ca2+ current in the neurons was observed in mouse model of absence epilepsy, our previous Ca2+ imaging studies also suggested that calcium overloading in the hippocampus CA3 neurons of kindling rats was correlated with the epileptogenesis[2]. Mn2+ has an ionic radius similar to that of Ca2+ , and it is handled in a similar to Ca2+ in many biological systems. Mn2+ is known to enter cells through calcium pathways such as voltage-gated calcium channels and microtubules transmission systems[3], Mn2+ is paramagnetic and can act as an excellent MRI contrast agent to direct imaging of brain function, it is a new promptly developing technique of brain imaging. Yi-Jen Lin[4] et al's studies using this technique have showed that activation of the brain with glutamate led to increase in MRI signal in the brain to 238±23% of the original. Different central manifestations in response to electro acupuncture at analgesic and no analgesic acupoints in rats were observed by manganese-enhanced functional MRI study[5]. Aoki [6] et al made middle cerebral artery embolism models, early ischemic region was observed on this model by use of Mn2+-fMRI. But at present there is no report about the Mn2+-fMRI of epilepsy.Pathological foundation of epilepsy is the neuronic massive discharge, as for this discharge is now presumed to be briefly Volant Ca2+ influx and cellular depolarization caused by slow Ca2+ influx. Because PTZ can induce excitatory amino acids(EAA) release, then EAA combines with its receptor, which causes calcium channels open, so calcium influx is enhanced, this event c
机译：目的：我们以前的研究表明钙超载与癫痫发生有关。在许多生物系统中，Mn2 +的处理方式类似于Ca2 +，并且可以作为出色的MRI造影剂，因此我们进行了本研究，以通过Mn2 + -fMRI确定与癫痫发生相关的脑区域，并进一步确定癫痫与钙超载。方法将猫分为两组，第一组分为A组和B组，A组为PTZ实验组，B组为生理盐水（NS）对照组，第一组用于脑电图检查，成年雄性。猫用氯胺酮和氯德拉嗪麻醉，肌内注射PTZ（55mg / kg），观察其行为学和脑电图变化。第二组分为A组和B组，A组用于急性癫痫发作的Mn2 + -fMRI，B组在急性癫痫发作诱发的24小时后用于Mn2 + -fMRI，C组为急性癫痫发作。对照组NS。将成年雄性猫与氯胺酮和氯达拉嗪联合麻醉;取两个股静脉，一个用于Mncl2输注，另一个用于甘露醇输注。给这些猫肌肉注射PTZ，并在TI加权MRI下观察其变化。去除信号增强型脑区域。结果：肌肉注射PTZ引起的猫的成猫率为80％，脑电图呈阵发性，高幅尖峰-慢波，对照组的病因和脑电图正常。当猫表现为全身性强直-阵挛性惊厥发作时，大脑皮层显示弥散性信号增强，额顶叶枕叶增强率为34.6;颞叶增强率为22.9，与对照组相比差异有统计学意义。癫痫发作发作后24小时的Mn2 + -fMRI也显示额叶顶叶信号增强。脑区域增强的神经元表现出明显的变性和坏死。结论肌内PTZ（55mg / kg）可以成功地诱发猫。额叶顶叶是癫痫的相关脑区，Mn2 + -fMRI在癫痫发作的定位和揭示发病机理中具有重要作用。引言Felix等[1]已证明在失神癫痫的小鼠模型中神经元中的Ca2 +电流增加，我们先前的Ca2 +影像学研究还表明，点燃大鼠海马CA3神经元中的钙超载与癫痫发生有关[ 2]。 Mn2 +具有类似于Ca2 +的离子半径，并且在许多生物系统中都以类似于Ca2 +的方式处理。众所周知，Mn2 +是通过钙通道（如电压门控钙通道和微管传输系统）进入细胞的[3]，Mn2 +具有顺磁性，可以作为直接对脑功能成像的出色的MRI造影剂，是一种迅速发展的新技术影像学Lin Yi-Jen Lin [4]等人的研究表明，使用谷氨酸激活大脑会导致脑部MRI信号增加至原始238±23％。锰增强功能性MRI研究观察到镇痛对电针反应的不同主要表现，并且没有观察到镇痛点[5]。 Aoki [6]等人建立了大脑中动脉栓塞模型，使用Mn2 + -fMRI在该模型上观察到了早期缺血区域。但目前尚无有关癫痫的Mn2 + -fMRI的报道。癫痫的病理学基础是神经质大量放电，因为现在推测这种放电是短暂的Volant Ca2 +流入和缓慢的Ca2 +流入引起的细胞去极化。由于PTZ可以诱导兴奋性氨基酸（EAA）释放，因此EAA与它的受体结合，导致钙通道开放，因此钙内流增强，此事件

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