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首页> 外文期刊>The Internet Journal of Nutrition and Wellness >Protective role of tea polyphenol, EGCG, against genotoxic damage induced by anticancer drugs and steroid compounds in cultured human lymphocytes
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Protective role of tea polyphenol, EGCG, against genotoxic damage induced by anticancer drugs and steroid compounds in cultured human lymphocytes

机译：茶多酚EGCG对培养的人淋巴细胞中抗癌药和类固醇化合物引起的遗传毒性损伤的保护作用

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Tea (Camellia sinensis) is a rich source of polyphenols called flavonoids, effective antioxidants found throughout the plant kingdom. The slight astringent, bitter taste of green tea is attributed to polyphenols. A group of flavonoids in green tea are known as catechins, which are quickly absorbed into the body and are thought to contribute to some of the potential health benefits of tea. The fresh tea leaves contain four major catechins as colourless water soluble compounds. epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC) and epigallocatechin gallate (EGCG). In this study we seen the antigenotoxic effect of tea polyphenol Epigallocatechin gallate (EGCG), against genotoxic damage induced by a steroid Trenbolone and an anticancer drug Docetaxel in cultured human lymphocytes, both in absence and presence of metabolic activation. Introduction EGCG makes up about 10-50% of the total catechin content and appears to be the most powerful of the catechins. In green tea catechin levels ranged from 51.5 - 84.3 mg/g, with epigallocatechin gallate (EGCG) being the main catechin in Chinese and Indian green teas. (EGCG) epigallocatechin gallate, a major catechin of found in green tea, has possible role in chemoprevention and chemotherapy of various types of cancers mainly prostate cancer (1, 2) and colon cancer (3, 4). EGCG is found to be the major and most photoprotective polyphenolic component of green tea (5). EGCG also induces growth arrest and apoptosis through multiple mechanisms, and can be used for cancer prevention, mainly pancreatic (6). Tea catechins and polyphenols are effective scavengers of reactive oxygen species in vitro and may also function indirectly as antioxidants through their effects on transcription factors and enzyme activities (7).Trenbolone is a synthetic steroid used frequently by veterinarians on livestock as a promoter of growth in animal husbandry (8). Trenbolone compounds have not yet been approved by the Food and Drug Administration, USA for use by humans due to their considerable negative side effects, although bodybuilders use the drug illegally to increase body mass and strength. Cases of prostate and hepatic cancers have been associated with long term anabolic steroid abuse (9,10). Trenbolone compounds increase nitrogen uptake by muscles after metabolization, leading to increased rate of protein synthesis. Docetaxel is a clinically well established anti-mitotic chemotherapy medication used mainly for the treatment of breast, ovarian, and non-small cell lung cancer (11) Docetaxel has an approved claim for treatment of patients who have locally advanced, or metastatic breast or non small-cell lung cancer who have undergone anthracycline-based chemotherapy and failed to stop cancer progression or relapsed. Docetaxel is marketed worldwide under the name Taxotere by Sanofi-Aventis. Evaluation of docetaxel pharmacokinetics in phase II and III clinical studies were with 100 mg/m2 dosages given over one-hour infusions every three weeks Docetaxel is a chemotherapeutic agent and is a cytotoxic compound and so is effectively a biologically damaging drug (12). Because docetaxel is a cell cycle specific agent, it is cytotoxic to all dividing cells in the body.EGCG was studied for its antimutagenic effect on the CAs and SCEs induced by Trenbolone and Docetaxel, both in the presence and absence of metabolic activation system in human lymphocytes in vitro. The readily quantifiable nature of sister chromatid exchanges with high sensitivity for revealing toxicant-DNA interaction and the demonstrated ability of genotoxic chemicals to induce significant increase in sister chromatid exchanges in cultured cells has resulted in this endpoint being used as indicator of DNA damage in blood lymphocytes of individuals exposed to genotoxic carcinogens (13). The above genotoxic endpoints are well known markers of genotoxicity and any reduction in the frequency of these genotoxic endpoints gives us indication of t
机译：茶（Camellia sinensis）是称为黄酮类化合物的多酚的丰富来源，黄酮类化合物是遍布整个植物界的有效抗氧化剂。绿茶的轻微涩味和苦味归因于多酚。绿茶中的一组类黄酮称为儿茶素，可迅速吸收到体内，并被认为对茶的某些潜在健康有益。新鲜茶叶含有四种主要的儿茶素，为无色水溶性化合物。表儿茶素（EC），表儿茶素没食子酸酯（ECG），表没食子儿茶素（EGC）和表没食子儿茶素没食子酸酯（EGCG）。在这项研究中，我们观察到茶多酚表没食子儿茶素没食子酸酯（EGCG）对由类固醇群勃龙和抗癌药多西紫杉醇在培养的人淋巴细胞中引起的遗传毒性损害的抗原毒性作用，无论是否存在代谢活化。简介EGCG约占儿茶素总量的10-50％，似乎是最强大的儿茶素。绿茶中的儿茶素含量范围为51.5-84.3 mg / g，表没食子儿茶素没食子酸酯（EGCG）是中国和印度绿茶中的主要儿茶素。表没食子儿茶素没食子酸酯（EGCG）是绿茶中的主要儿茶素，在化学预防和化学治疗多种癌症中可能发挥作用，这些癌症主要是前列腺癌（1、2）和结肠癌（3、4）。 EGCG被发现是绿茶的主要和最具光保护性的多酚成分（5）。 EGCG还通过多种机制诱导生长停滞和凋亡，可用于癌症预防，主要是胰腺癌（6）。茶儿茶素和多酚是体外活性氧的有效清除剂，还可以通过其对转录因子和酶活性的影响而间接用作抗氧化剂（7）。群勃龙是兽医在牲畜上经常使用的合成类固醇，它是家畜生长的促进剂。畜牧业（8）。群勃龙化合物由于其相当大的副作用而尚未被美国食品药品监督管理局批准用于人类，尽管健美者非法使用该药物来增加体重和体力。前列腺癌和肝癌病例与长期合成代谢类固醇滥用有关（9,10）。群勃龙化合物增加了代谢后肌肉对氮的吸收，从而导致蛋白质合成速率增加。多西他赛是临床上公认的抗有丝分裂化学疗法药物，主要用于治疗乳腺癌，卵巢癌和非小细胞肺癌（11）多西他赛已获批准用于治疗局部晚期或转移性乳腺癌或非乳腺癌的患者。小细胞肺癌，已接受基于蒽环类药物的化学疗法，但未能阻止癌症进展或复发。赛诺菲-安万特公司（Dooftaxel）以赛得利（Taxotere）的名称在全球范围内销售。多西紫杉醇在II期和III期临床研究中的药代动力学评估是每三周输注一小时，每次100 mg / m2剂量。多西紫杉醇是一种化学治疗剂，是一种细胞毒性化合物，因此实际上是一种对生物有害的药物（12）。由于多西他赛是一种细胞周期特异性药物，因此对人体所有分裂细胞均具有细胞毒性。研究了EGCG对群勃龙和多西他赛诱导的CAs和SCEs的抗诱变作用，无论是否存在人体代谢激活系统体外淋巴细胞。姊妹染色单体交换具有可定量的性质，对揭示毒物-DNA相互作用具有很高的敏感性，并且已证明遗传毒性化学物质诱导培养细胞中姊妹染色单体交换显着增加的能力已导致该终点被用作血液淋巴细胞DNA损伤的指标暴露于遗传毒性致癌物的个体数量（13）。上述遗传毒性终点是众所周知的遗传毒性标记，这些遗传毒性终点频率的任何降低都为我们指示了

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