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首页> 外文期刊>The Internet Journal of Orthopedic Surgery >Immunochromatographic detection of Helicobacter pylori antibodies in paediatric patients in Asaba, Nigeria.
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Immunochromatographic detection of Helicobacter pylori antibodies in paediatric patients in Asaba, Nigeria.

机译：尼日利亚Asaba儿科患者的幽门螺杆菌抗体的免疫色谱检测。

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Background: Helicobacter pylori has been implicated as one of the aetiological agents of gastrointestinal infections in children in developing countries. Paediatric patients attending Federal Medical Centre (FM?) and General Hospital (GH) Asaba, Nigeria, were serologically assessed for the infection.Methodology: 100 blood samples were aseptically collected by venepuncture from subjects and controls, and screened for Helicobacter pylori antibodies, using an immunochromatographic assay method.Results: An overall high prevalence rate of 55% was recorded. At the FMC, 8(20%) and 2(20%) prevalence was recorded for subjects and controls respectively, while at the GH, 14(35%) and 4(40%) were seropositive for the subjects and controls respectively.Conclusion: A causal relationship between gastrointestinal complaints and Helicobacter infection as well as asymptomatic carriage of infection amongst children in the study area has been serologically demonstrated. There is urgent need for routine screening of paediatric patients presenting with abdominal pains. Introduction The taxonomy of the genus Campylobacter went through extensive revision in the late 1980s. The organism, Helicobacter pylori is a Gram negative curved bacillus that colonizes gastric mucosa in humans. It was first isolated from a gastric mucosa biopsy specimen in 1983 [1]. It plays an important role in the development of peptic ulcer disease [2,3]. About half of the adults may be infected with this pathogen in developed countries while this figure could be as high as 90% in developing countries [4]. Predisposing factors such as overcrowding of people, poor sanitation and living conditions can be found in most developing countries. [4] have reported that close person to person contact in childhood is an important determinant of seroprevalence of H. pylori in adulthood, suggesting that the infection is transmitted directly from one person to another and may be commonly acquired in early life. The helicobacters are one of the major bacterial causes of enteritis worldwide and their prevalence makes them important from both clinical and economic perspectives. Accurate identification of these organisms is therefore a desirable objective, to provide important clinical and epidemiological information in a developing country such as Nigeria.Several diagnostic methods such as the pulsed-field-gel- electrophoresis [5], PCR [6], protein profiling [7] etc, have been used. These methods, no doubt, require special technologies, high cost and trained manpower factors that put them out of the ready reach of routine laboratory use in most third world countries. While the combination of culture and histological examination of gastric biopsy specimens has been considered the “gold standard” for the diagnosis of Helicobacter pylori, serological diagnosis has also been proven to be more sensitive [8,9]. All patients colonized with H. pylori elicit a local antibody response against antigens covering the surface and flagella of the organism. In the majority of the cases this antibody response is detectable in the serum. These circulating antibodies can be detected by a variety of techniques. Currently, the majority of routine serodiagnostic tests for H. pylori measure lgG antibodies. Locally, the antibodies are mostly of the lgA class, but the circulating antibodies are primarily of the lgG class, usually the lgGI, lgG2) and lgG4 subclasses. [10]. A specific lgG response at least as great as that found in adults can also be detected in infected children [11]. Here we report the prevalence of Helicobacter pylori antibodies among children within Asaba, a busy metropolitan city in Delta region of Nigeria. The objective of study was also to assess the evidence for a cause – and – effect relationship between H. pylori infection and gastrointestinal complaints among children visiting two major hospitals in Asaba. Subjects and Methods The subjects comprised of 100 children (aged 1 year – 16 years), who
机译：背景：幽门螺杆菌被认为是发展中国家儿童胃肠道感染的病因之一。血清学评估了在尼日利亚联邦联邦医疗中心（FM？）和总医院（GH）阿萨巴的儿科患者的感染情况。方法：通过静脉穿刺术从受试者和对照中无菌采集100份血液样本，并使用筛选幽门螺杆菌抗体结果：总体患病率高达55％。在FMC上，受试者和对照组的患病率分别为8（20％）和2（20％），而在GH上，受试者和对照组的患病率分别为14（35％）和4（40％）。 ：已通过血清学证明了胃肠道不适与幽门螺杆菌感染之间的因果关系以及无症状感染在儿童中的因果关系。迫切需要常规筛查出现腹痛的儿科患者。简介弯曲杆菌属的分类在1980年代后期经历了广泛的修订。幽门螺杆菌是一种革兰氏阴性弯曲杆菌，可在人的胃粘膜上定居。它最早是在1983年从胃粘膜活检标本中分离出来的[1]。它在消化性溃疡疾病的发展中起重要作用[2,3]。在发达国家，大约一半的成年人可能感染了这种病原体，而在发展中国家，这一数字可能高达90％[4]。在大多数发展中国家，容易发现人满为患，卫生条件差和生活条件等诱因。 [4]报告说，儿童时期人与人的密切接触是成人中幽门螺杆菌血清阳性率的重要决定因素，这表明感染是直接从一个人传播到另一个人的，并且可能是在早期生活中普遍获得的。幽门螺杆菌是全世界肠炎的主要细菌原因之一，其流行使得从临床和经济角度来看它们都很重要。因此，准确鉴定这些生物体是一个理想的目标，以便在诸如尼日利亚的发展中国家提供重要的临床和流行病学信息。多种诊断方法，例如脉冲场凝胶电泳[5]，PCR [6]，蛋白质谱分析[7]等已被使用。毫无疑问，这些方法需要特殊的技术，高成本和受过训练的人力因素，这使它们无法在大多数第三世界国家中进行常规实验室使用。虽然将胃活检标本的培养和组织学检查相结合已被认为是诊断幽门螺杆菌的“金标准”，但血清学诊断也被证明更加敏感[8,9]。幽门螺杆菌定植的所有患者均引起针对覆盖生物体表面和鞭毛的抗原的局部抗体反应。在大多数情况下，这种抗体反应在血清中是可检测的。可以通过多种技术检测这些循环抗体。目前，大多数针对幽门螺杆菌的血清学常规诊断方法可检测IgG抗体。就局部而言，抗体大部分属于IgGA类，但是循环抗体主要属于IgG类，通常是IgG1，IgG2和IgG4亚类。 [10]。在感染的儿童中也可以检测到至少与成年人相同的特异性IgG反应[11]。在这里，我们报道了尼日利亚三角洲繁忙城市阿萨巴（Asaba）内儿童中幽门螺杆菌抗体的患病率。该研究的目的还在于评估前往Asaba两家主要医院的儿童中幽门螺杆菌感染与胃肠道不适之间因果关系的证据。对象和方法对象由100名儿童（1岁至16岁）组成，他们

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