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首页> 外文期刊>The Internet journal of parasitic diseases >Larvae of Strongyloides stercoralis in the Venous Blood of a 25 Year Old Female Patient at the National Hospital, Abuja Nigeria
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Larvae of Strongyloides stercoralis in the Venous Blood of a 25 Year Old Female Patient at the National Hospital, Abuja Nigeria

机译：尼日利亚阿布贾国家医院一名25岁女性患者静脉血中的类固醇幼虫幼虫

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A 25 year-old female patient with a history of recurrent pruritus, particularly especially in the evenings over the course of about six years, was seen at the Family Medicine Clinic, National Hospital, Abuja Nigeria in April, 2008. Both diurnal and nocturnal blood samples of the patient were analyzed using 2% formol water and stained with 1% Giemsa stain for 30 minutes. The result was an unusual occurrence. While the diurnal samples had microfilariae of Mansonella species, the nocturnal samples in addition to microfilariae of Mansonella species also had larvae of Strongyloides stercoralis. This paper is to draw the attention of practicing biomedical scientists of unusual results while processing specimens in our laboratories. Introduction A 25 year-old female patient with a history of recurrent pruritus, particularly especially in the evenings over the course of about six years, was seen at the Family Medicine Clinic, National Hospital, Abuja. According to the patient, the problem started when she was living in Lagos. She now lives in Jos while schooling at Yola, Adamawa State. This patient was sent to the Parasitology laboratory for blood filariasis test. Both diurnal and nocturnal blood samples of the patient were analyzed at the Parasitology laboratory. Materials And Method MaterialsCollection Of Blood2 ml of both diurnal and nocturnal venous blood samples were collected from the patient into 2% sodium citrate solution. While the diurnal sample was collected at about 12:00 noon (10:00-2:00pm), the nocturnal sample was collected at about 10:00 pm (10:00pm – 2:00am).Method For Examination Of Haemolysed Venous BloodConcentration methodml of venous blood was added to 9 ml of 2 % formalin solution in a tube; mixed and allowed to stand for 5 – 10 minutes for the red cells to haemolyse. The blood / formalin mixture was centrifuged at 1000 rpm for I0 minutes. The supernatant fluid was discarded and the deposit resuspended. The deposit was transferred to a slide to form a thin smear and the preparation examined microscopically at 10 x and 40 x objectives. Positive slides are allowed to dry in the air overnight and stained with 1 % Giemsa stain for 30 - 45 minutes. The stained slide is then washed with water and allowed to air dry. Th examination of stained slide was with 100 x objective. Result Both the diurnal and nocturnal blood samples had microfilariae of Mansonella species. The nocturnal blood sample also had larvae of Strongyloides stercoralis.
机译：2008年4月，在尼日利亚阿布贾国家医院的家庭医学诊所内，发现了一名25岁的女性患者，该患者有反复瘙痒的病史，尤其是在大约六年的傍晚时分。昼夜血液和夜间血液使用2％的甲醛水对患者的样品进行分析，并用1％的吉姆萨染色剂染色30分钟。结果是不寻常的事件。尽管昼夜样品具有曼森氏菌属的微丝虫病，但夜间样品中除了曼森氏菌属的微丝虫病外，还具有硬实线虫的幼虫。本文旨在在实验室中处理标本的同时，引起实践中异常结果的生物医学科学家的注意。引言在阿布贾国家医院的家庭医学诊所，发现了一位25岁的女性患者，该患者有反复瘙痒的病史，尤其是在大约六年的晚上。据病人说，问题开始于她住在拉各斯。她现在在阿达玛瓦州约拉学习时住在乔斯。该患者被送至寄生虫学实验室进行血丝虫病检测。在寄生虫学实验室对患者的昼夜血样进行了分析。材料和方法材料血液收集从患者收集2毫升昼夜静脉血样本到2％柠檬酸钠溶液中。白天的样本大约在中午12:00（10：00-2：00pm）采集，而夜间样本则在大约10:00 pm（10:00 pm – 2:00 am）采集。血液溶血静脉血浓度的检测方法方法将静脉血1ml加到9ml 2％福尔马林溶液中。混合，静置5-10分钟，使红细胞溶血。将血液/福尔马林混合物以1000rpm离心10分钟。丢弃上清液，并将沉淀物重新悬浮。将沉积物转移至载玻片上以形成薄涂片，并在10 x和40 x物镜下显微镜检查制剂。使阳性玻片在空气中干燥过夜，并用1％Giemsa染色剂染色30-45分钟。然后将染色的载玻片用水洗涤并风干。染色载玻片的检查是用100×物镜进行的。结果夜间和夜间血样均具有曼森氏菌属的微丝虫病。夜间血样中还含有小钩虫（Strongyloides stercoralis）的幼虫。

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