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Flavobacterium panaciterrae sp. nov., a β-glucosidase producing bacterium with ginsenoside-converting activity isolated from the soil of a ginseng field

机译:泛黄杆菌nov。,一种从人参田土壤中分离出的具有人参皂苷转化活性的产生β-葡萄糖苷酶的细菌

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DCY69T is JX233806. A Gram-reaction-negative, oxidase- and catalase-positive, non-gliding motile strain, designated strain DCY69T, was isolated from the soil of a ginseng field in the Republic of Korea. Colonies of strain DCY69T were circular, 0.5a?’1.5 mm diameter, yellow, and convex on an R2A agar plate after 2 days. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DCY69T belonged to the genus Flavobacterium with 90.5a?’98.3% gene sequence similarity. The major predominant quinone was MK-6. The major cellular fatty acids were iso-C15:0, iso-C17:0 3-OH, iso-C15:0 3-OH and summed feature 3 (containing C16:1?‰7c and/or C16:1?‰6c). The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and unidentified polar lipids (L1, L2). The genomic DNA G+C content of strain DCY69T was 35.0mol%. The strain DCY69T transformed ginsenoside Rb1 into Rd and F2. Based on the polyphasic taxonomic data, strain DCY69T is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium panaciterrae sp. nov. is proposed. The type strain is DCY69T(= KCTC 32392T = JCM 19161T), isolated from the soil of a ginseng field in the Republic of Korea.
机译:DCY69T菌株16S rRNA基因序列的GenBank / EMBL / DDBJ登录号为JX233806。从大韩民国的人参田地土壤中分离出革兰氏反应阴性,氧化酶和过氧化氢酶阳性,不滑动的运动菌株,称为菌株DCY69T。 DCY69T菌株的菌落为圆形,直径0.5a?1.5毫米,黄色,在2天后在R2A琼脂平板上凸出。基于16S rRNA基因序列的系统进化分析表明,菌株DCY69T属于黄杆菌属,具有90.5a?98.3%的基因序列相似性。主要的主要醌是MK-6。主要的细胞脂肪酸为iso-C15:0,iso-C17:0 3-OH,iso-C15:0 3-OH和特征3的总和(包含C16:1?‰7c和/或C16:1?‰6c )。主要的极性脂质是磷脂酰乙醇胺,一种未鉴定的氨基脂质和未鉴定的极性脂质(L1,L2)。 DCY69T菌株的基因组DNA G + C含量为35.0mol%。 DCY69T菌株将人参皂甙Rb1转化为Rd和F2。根据多相分类学数据,菌株DCY69T被认为代表黄杆菌属的一个新物种,其名称为Panociterrae sp.。十一月被提议。从大韩民国的人参田土壤中分离出来的菌株是DCY69T(= KCTC 32392T = JCM 19161T)。

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