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A Novel Method of Identifying Micro & Macro Filarial Nematodes

机译：一种识别宏观和宏观丝状线虫的新方法

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Introduction The recent outbreak of Wuchereriasis Bancrofti infections in Southern areas of Tamil Nadu has resulted in a number of various manifestations in all age groups and both sexes irrespective of economic status and sanitary conditions. The onset of monsoon coincides with heavy mosquito spread both Anopheles and Culex species in all areas of localities. The range of manifestations starts from cutaneous, lymphatic, serous cavities and various other systems also. The pediatric age group is particularly vulnerable so also the rest of community. Clinical Study The picture of Wuchereriasis range from itchy dermatitis, lymphorrhea to pulmonary symptoms, central nervous system involvement, cardiac picture with chest pain, dyspnea and gastrointestinal pain, vomiting, fever and gentiurinary picture of severe dysuria, hematuria, chyluria, dysmonorrhea., abortions, still births and suspected intrauterine transfer to foetus also. Misnomers A number of cases missed and wrongly diagnosed and treated as tuberculosis, meningo-encephalitis, malignancies, myocardial Infarcts, asthmas, arthritis.. Common Pitfalls of Diagnosis The time honored teaching of peripheral blood smear study should be done to coincide with periodicity – nocturnal after 10 p.m to 2 a.m has resulted in missing so many cases of Filariasis in all localities. Our study aimed at finding the parasites in peripheral blood and saliva, urine, sputum, serosanguinous discharges, semen, vaginal fluids and CSF. Methodology A novel PVC sheet treated with Na3N salt acts as a very good medium to trap, entice the parasites both Micro & Macro Filariae. The same PVC sheet gives blue coloration to Male and red color to Female adult parasites in blood, tissues and various other fluids. The treated PVC sheet used as a cover slip gives positive results done at any time of the day both day and night. The other old method of Wright’s, Giesma stain were not needed here. The technique is able to preserve the specimen for more than 48 hours also. Conclusion The tropical disease masquerading in Asian, African continents wreaking havoc in local populations has to be tackled on a war footing. The identification of cases both occult and clinical can be very much enhanced to a great extent both technically and financially simpler with this new treated PVC technique. Clinical Photographs
机译：简介最近在泰米尔纳德邦南部地区爆发的班氏支原体（Wuchereriasis Bancrofti）感染已导致各个年龄段和性别的各种表现形式，无论经济状况和卫生状况如何。季风的爆发与蚊子在当地的所有地区传播的按蚊和库蚊种类相吻合。表现范围从皮肤，淋巴，浆液腔和各种其他系统开始。小儿年龄段尤其容易受到伤害，其他社区也是如此。临床研究Wuchereriasis的图像范围从瘙痒性皮炎，淋巴出血到肺部症状，中枢神经系统受累，心脏图像伴有胸痛，呼吸困难和胃肠道疼痛，呕吐，发烧和泌尿生殖系统症状，严重排尿困难，血尿，乳糜尿，痢疾，流产。 ，死胎和疑似宫内转移到胎儿也。错误的误诊许多病例被误诊和误诊为结核病，脑膜脑炎，恶性肿瘤，心肌梗塞，哮喘，关节炎。。常见的诊断误区应当及时进行外周血涂片检查，以符合周期性–夜间晚上10点至凌晨2点之后，导致各地都有如此多的丝虫病病例失踪。我们的研究旨在发现外周血和唾液，尿液，痰，血性排泄物，精液，阴道液和脑脊液中的寄生虫。方法论一种新型的用Na3N盐处理过的PVC片材是一种很好的诱集媒介，诱使Micro和Macro Filariae的寄生虫进入。相同的PVC片会使血液，组织和其他各种液体中的雄性成虫寄生虫呈蓝色，雌性成虫呈红色。用作盖玻片的经过处理的PVC片材在白天和黑夜的任何时间都可获得积极的效果。莱特的另一种旧方法是吉斯玛染色，这里不需要。该技术还能够将样本保存超过48小时。结论必须在战争的基础上解决在亚洲，非洲大陆伪装的热带疾病，给当地居民造成严重破坏。借助这种新的处理过的PVC技术，可以在技术上和财务上大大简化对隐匿和临床病例的识别。临床照片

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