...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Veterinary Medical Science >Hormonal Differences in Peripheral Blood and Gene Profiling in the Liver and Lymphocytes in Japanese Black Cattle with Growth Retardation
【24h】

Hormonal Differences in Peripheral Blood and Gene Profiling in the Liver and Lymphocytes in Japanese Black Cattle with Growth Retardation

机译：生长发育迟缓的日本黑牛的外周血激素水平差异和肝，淋巴细胞基因谱分析

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

References(30) Cited-By(1) Japanese Black cattle occasionally demonstrate growth retardation despite sufficient nutrient intake. To clarify hormonal and transcriptional characteristics, we investigated differences in blood components, including hormones, and differences in exhaustive gene expressions in the liver and peripheral lymphocytes of six cattle with growth retardation (GR cattle) and eight control cattle of the same age and pedigree with normal growth. Hematocrit values and concentrations of hemoglobin, serum albumin, total cholesterol, insulin-like growth factor 1 (IGF-1), thyroxine and insulin in GR cattle were significantly lower than those in controls. GR cattle also excreted higher levels of GH. We used three GR and three control cattle for a microarray analysis in the liver and found that 279 gene expressions were significantly different. However, gene expressions related to the GH-IGF-1 axis, such as the GH receptor and IGF-1, were not significantly different from those of controls. Immune-related gene expressions were significantly lower. To clarify these gene expression levels, peripheral lymphocytes were used for real-time RT-PCR. The expression rates of genes that were significantly lower in the liver, such as chemokine ligand 8, interferon gamma receptor 1 and immunoglobulin light chain VJ region were also significantly lower in three GR cattle than those in the three control cattle. These results suggest that the cause of growth retardation in the present study was due to other factors, not abnormal gene expressions of factors related to the GH-IGF-1 axis in the liver, and that GR cattle were susceptible to infectious disease.
机译：参考文献（30）被引（1）日本黑牛偶尔会表现出生长迟缓，尽管摄入了足够的养分。为了弄清激素和转录特性，我们调查了六只生长迟缓的牛（GR牛）和八只年龄相同，血统不同的对照牛的血液成分（包括激素）的差异以及肝脏和外周淋巴细胞中力竭基因表达的差异。正常增长。 GR牛的血细胞比容值和血红蛋白，血清白蛋白，总胆固醇，胰岛素样生长因子1（IGF-1），甲状腺素和胰岛素的浓度均显着低于对照组。 GR牛也排泄了较高水平的GH。我们使用三只GR和三只对照牛在肝脏中进行微阵列分析，发现279个基因表达有显着差异。但是，与GH-IGF-1轴相关的基因表达，例如GH受体和IGF-1，与对照组相比没有显着差异。免疫相关基因的表达明显降低。为了阐明这些基因表达水平，将外周淋巴细胞用于实时RT-PCR。在三个GR牛中，肝脏中趋化因子配体8，干扰素γ受体1和免疫球蛋白轻链VJ区等肝脏中显着较低的基因的表达率也显着低于三个对照牛。这些结果表明，本研究中生长迟缓的原因是由于其他因素引起的，而不是肝脏中与GH-IGF-1轴相关的因素的基因表达异常，并且GR牛容易感染传染病。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号