Antiulcerogenic activities of the Methanolic extract of Cissus quadrangularis in wistar

G. Shanthi; G. Vijay kanth; L. Hitesh; M. Ganesan

摘要

Cissus quadrangularis (family: Vitaceae) is one of the species widely used in folk medicine to hasten the fracture healing process. The methanolic extract of Cissus quadrangularis (CQE) was investigated to evaluate its anti-ulcer activity by using aspirin induced gastric ulcer model, since non-steroidal anti-inflammatory drugs (such as aspirin) causes gastrointestinal damages (ulcers) as one of their side effects in human. CQE was administered at the dose of 1000 mg/Kg.b.w to the groups of wistar rat orally for 7 consecutive days, after which gastric ulcer was induced by aspirin. Gross pathological examination reveals that CQE promotes ulcer protection by decreasing the ulcer index which is evident in the Histopathological observation and qualitative analysis of C-reactive protein. The CQE anti-ulcerogenic effect was compared with ranitidine (RTD) (30mg/Kg.b.w) found to have better protective effect. Introduction Always there has been renewed interest in identifying new antiulcer drugs from natural sources (Brito et al., 1997) since chemical compounds are known to have undesirable side-effects.Gastric ulcers are believed to be due to an imbalance between acid and pepsin along with weakness of the mucosal barrier. The gastric mucosa is continuously exposed to potential injurious agents such as acid, pepsin, bile acid, bacterial products and drugs (Goncalves et al., 2006). In recent times, many medicinal plants continue to provide valuable therapeutic agents for the treatment of ulcers both in modern medicine and by the traditional system throughout the world. Besides, some medicinal plants have been found to have both preventive and/or therapeutic effects on ulcers. The present work was focused to study the preventive effect of Cissus quadrangularis extract (CQE) against the ulcerative lesions caused by aspirin on wistar rat by gross pathological examination, histopathological examination and C- reactive protein analysis. Cissus quadrangular is Cissus quadrangularis Linn. belongs to a family- Vitaceae and are found in hotter parts of India, Ceylon, East Africa and Malaysia and Thailand. It is commonly known as “bone setter” since it has the fracture healing property and are used as a food supplement in southern India. For this study the plant (stem region) was collected from Mayavaram, Tamilnadu, India and was duly authenticated by Dr.Sasikala Ethirajulu, Dept. of Pharmacognosy, Central Research Institute for Siddha, Arumbakkam, Chennai- 06. Materials and methods Extract PreparationThe collected plant stem was shade dried and coarsely powdered .About 1 kg of the powdered material was soaked in methanol for 48 h and extracted by soxhlet extraction. The extract was vacuum dried and was stored at ?4?C until further use. AnimalsMale albino Wistar rats weighing 120–150 g, (Source: Micro Therapeutic Research Labs, Pvt, Ltd, Chennai) were acclimatized for 7 days and randomized into 4 groups of six each. Animals were housed in polypropylene cages with 12h light and 12h dark photoperiod. Temperature and humidity were maintained between 18 and 25 ?C and 30 and 70 % respectively. Animals were supplied with standard pelleted feed and filtered water ad libitum. The experiment was conducted as per CPCSEA guidelines and the study was approved by the Institutional Animal Ethical committee.Treatment protocolOf the 4 randomized groups, group I - Control animals received only the corn oil; Group II -Animals received aspirin (400 mg/kg.b.w). Where as the group III and IV animals were treated with ranitidine (30 mg/kg.b.w.) and CQE (1000 mg/kg.b.w.) respectively for seven days orally. After last dosing, all animals were fasted for 16h and then group III and group IV animals treated with ranitidine (30 mg/kg.b.w.), CQE (1000 mg/kg.b.w.) respectively. Thirty minutes later Group II, III and IV animals were treated with Aspirin (400 mg/kg) orally. After 6 h all animals were sacrificed and subjected for anti- ulcerative effect. The maximum dose volume o

机译：四角形犬（Cussus quadrangularis）（科：Vi科）是广泛用于民间医学中以加速骨折愈合过程的物种之一。由于非甾体类抗炎药（例如阿司匹林）会引起胃肠道损害（溃疡），因此，使用阿司匹林诱导的胃溃疡模型研究了四叶草的甲醇提取物（CQE）以评估其抗溃疡活性。对人类的影响。连续7天对Wistar大鼠各组口服1000mg / Kg.b.w的CQE，然后阿司匹林诱发胃溃疡。大体病理学检查显示，CQE通过降低溃疡指数来促进溃疡保护，这在组织病理学观察和C反应蛋白的定性分析中很明显。将CQE的抗溃疡作用与雷尼替丁（RTD）（30mg / Kg.b.w）进行比较，发现具有更好的保护作用。引言人们一直对从自然来源鉴定新的抗溃疡药一直抱有新的兴趣（Brito等，1997），因为已知化学化合物具有不良的副作用。胃溃疡被认为是由于酸和胃蛋白酶之间的不平衡引起的。粘膜屏障薄弱。胃粘膜持续暴露于潜在的伤害性物质，例如酸，胃蛋白酶，胆汁酸，细菌产品和药物（Goncalves等，2006）。近年来，无论是现代医学还是世界范围内的传统系统，许多药用植物都继续为治疗溃疡提供有价值的治疗剂。此外，已经发现一些药用植物对溃疡具有预防和/或治疗作用。本研究的重点是通过大体病理学检查，组织病理学检查和C反应蛋白分析研究四叶草提取物（CQE）对阿司匹林对wistar大鼠溃疡性病变的预防作用。四角形是Cissus quadrangularis Linn。属于蔷薇科，在印度，锡兰，东非以及马来西亚和泰国的较热地区发现。由于它具有骨折愈合性能，并且在印度南部被用作食品补充剂，因此通常被称为“骨结石器”。在本研究中，该植物（茎区域）是从印度泰米尔纳德邦Mayavaram收集的，并由印度钦奈阿鲁巴卡姆市锡德中央研究所药理学系Sasikala Ethirajulu博士正式认证。06.材料和方法提取物制备将植物茎进行阴凉干燥并粗粉。将约1 kg的粉状物质在甲醇中浸泡48小时，然后通过索氏提取法提取。将提取物真空干燥，并保存在约4℃下直至进一步使用。动物体重120–150 g（来源：Micro Therapeutic Research Labs，Pvt，Ltd，金奈）的雄性白化Wistar大鼠适应环境7天，随机分为4组，每组6组。将动物饲养在具有12h光照和12h黑暗光周期的聚丙烯笼中。温度和湿度分别保持在18至25°C和30至70％之间。给动物提供标准的颗粒饲料和随意的过滤水。实验按照CPCSEA指南进行，研究得到机构动物伦理委员会的批准。治疗方案在4个随机组中，第I组-对照动物仅接受玉米油;第4组为对照组。第二组-动物接受阿司匹林（400 mg / kg.b.w）。分别以雷尼替丁（30 mg / kg.b.w。）和CQE（1000 mg / kg.b.w。）对第III组和第IV组动物口服治疗7天。最后一次给药后，将所有动物禁食16小时，然后分别用雷尼替丁（30mg / kg.b.w。），CQE（1000mg / kg.b.w。）处理III组和IV组动物。三十分钟后，口服阿司匹林（400 mg / kg）治疗第二，第三和第四组动物。 6小时后，处死所有动物并进行抗溃疡作用。最大剂量体积o

Antiulcerogenic activities of the Methanolic extract of Cissus quadrangularis in wistar

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