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Chronologic change of serum IgG antibody response in chickens reinfected with Cryptosporidium baileyi

机译:百色隐孢子虫再感染鸡血清IgG抗体反应的年代学变化

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Eight 2-day-old SPF chickens were each inoculated orally with a single dose of 5×105 oocysts of Cryptosporidium baileyi, and immunoglobulin G (IgG) antibody responses were chronologically measured by indirect immunofluorescent antibody (IFA) assay. Anti-C. baileyi IgG antibody levels remained high(1:106.67 to 1:512.00) for at least 4 months with 330 days of a detectable period. Ten days after the negative conversion, each chicken was re-challenged with 1×107 oocysts of the same species. Subsequent infection in 340-day-old individuals caused sudden elevated IgG antibody levels and the titer peaked on day 28 postchallenge inoculation(PCI), at 1:1,024 with a 65 days of detection period. Chickens in primary infection showed oocyst shedding profiles, but did not exhibit any oocyst shedding before or after experimental reinfection.
机译:分别给八只2日龄SPF鸡口服单剂量的5×10 5 百色隐孢子虫卵囊,并通过间接免疫荧光抗体(IFA)按时间顺序测量免疫球蛋白G(IgG)抗体反应)分析。滑稽。百乐伊IgG抗体水平至少持续4个月(1:106.67至1:512.00),可检测的时间为330天。转化失败后的10天,每只鸡都用相同种类的1×10 7 卵囊进行了攻击。随后在340天的个体中感染导致IgG抗体水平突然升高,且效价在攻击后接种(PCI)第28天(1:1024)达到峰值,检测期为65天。初次感染的鸡表现出卵囊脱落特征,但在实验性再感染之前或之后均未表现出卵囊脱落。

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