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首页> 外文期刊>The Korean Journal of Parasitology >In Vitro Maintenance of Clonorchis sinensis Adult Worms
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In Vitro Maintenance of Clonorchis sinensis Adult Worms

机译：华支睾吸虫成虫的体外维持

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Clonorchis sinensis is a biological carcinogen inducing human cholangiocarcinoma, and clonorchiasis is one of the important endemic infectious diseases in East Asia. The present study investigated survival longevity of C. sinensis adult worms in various in vitro conditions to find the best way of keeping the worms longer. The worms were maintained in 0.85% NaCl, 1×PBS, 1×Locke's solution, RPMI-1640, DMEM, and IMDM media, and in 1×Locke's solution with different supplements. All of the worms died within 3 and 7 days in 0.85% NaCl and 1×PBS, respectively, but survived up to 57 days in 1×Locke's solution. The worms lived for 106 days in DMEM, and 114 days in both RPMI-1640 and IMDM media. The survival rate in RPMI-1640 medium was the highest (50%) compared to that in DMEM (20±10%) and in IMDM (33.3±25.2%) after 3 months. The 1×Locke's solution with 0.005% bovine bile supplement showed increased duration of maximum survival from 42 days to 70 days. Higher concentration of bile supplements than 0.005% or addition of glucose were disadvantageous for the worm survival. The worms died rapidly in solutions containing L-aspartic acid, L-glutamic acid, and adenine compared to L-arginine, L-serine, and L-tryptophan. In conclusion, the 1×Locke's solution best supports the worms alive among inorganic solutions for 57 days, and the RPMI-1640 medium maintains living C. sinensis adults better and longer up to 114 days in vitro than other media.
机译：中华支睾吸虫是一种生物致癌物，可诱发人类胆管癌，而支睾吸虫病是东亚地区重要的地方性传染病之一。本研究调查了中华绒螯蟹成虫在各种体外条件下的存活寿命，以找到使它们更长寿的最佳方法。蠕虫用0.85％NaCl，1×PBS，1×Locke溶液，RPMI-1640，DMEM和IMDM培养基以及1×Locke溶液中的不同补充剂饲养。所有蠕虫分别在0.85％NaCl和1×PBS中在3和7天内死亡，但在1×Locke溶液中存活了57天。蠕虫在DMEM中存活了106天，在RPMI-1640和IMDM介质中存活了114天。 3个月后，与DMEM（20±10％）和IMDM（33.3±25.2％）相比，RPMI-1640培养基的存活率最高（50％）。含0.005％牛胆汁的1×Locke溶液显示最大存活时间从42天增加到70天。胆汁补充剂的浓度高于0.005％或添加葡萄糖对蠕虫的存活不利。与L-精氨酸，L-丝氨酸和L-色氨酸相比，蠕虫在含有L-天冬氨酸，L-谷氨酸和腺嘌呤的溶液中迅速死亡。总而言之，1×Locke溶液可以最好地支持蠕虫在无机溶液中存活57天，而RPMI-1640培养基比其他培养基可以更好地维持中华绒螯蟹的成虫存活，并且在体外长达114天。

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