• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Veterinary Medical Science >Detection of in vivo DNA Damage Induced by Ethanol in Multiple Organs of Pregnant Mice Using the Alkaline Single Cell Gel Electrophoresis (Comet) Assay
【24h】

Detection of in vivo DNA Damage Induced by Ethanol in Multiple Organs of Pregnant Mice Using the Alkaline Single Cell Gel Electrophoresis (Comet) Assay

机译：碱性单细胞凝胶电泳（彗星）分析法检测乙醇对妊娠小鼠多器官的体内DNA损伤的检测

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

References(27) Cited-By(16) Ethanol is principal ingredient of alcohol beverage, but considered as human carcinogen, and has neurotoxicity. Alcohol consumption during pregnancy often causes fetal alcohol syndrome. The DNA damage is one of the important factors in carcinogenicity or teratogenicity. To detect the DNA damage induced by ethanol, we used an in vivo alkaline single cell gel electrophoresis (Comet) assay in pregnant mice organs and embryos. Pregnant ICR mice on Day 7 of gestation were treated with 2, 4 or 8 g/kg ethanol, and maternal organs/tissues and embryos were subjected to the Comet assay at 4, 8, 12 and 24 hr after ethanol treatment. Four and 8 g/kg ethanol induced DNA damage in brain, lung and embryos at 4 or 8 hr after the treatment. Two g/kg ethanol did not cause any DNA damage, and 8 g/kg ethanol only increased the duration of DNA damage without distinct increase in the degree of the damage. No significant DNA damage was observed in the liver. To detect the effect of acetaldehyde, disulfiram, acetaldehyde dehydrogenase inhibitor, was administered before 4 g/kg ethanol treatment. No significant increase of DNA damage was observed in the disulfiram pre-treated group. These data indicate that ethanol induces DNA damage, which might be related to ethanol toxicity. Since pre-treatment of disulfiram did not increase DNA damage, DNA damage observed in this study might not be the effect of acetaldehyde.
机译：参考文献（27）Cited-By（16）乙醇是酒精饮料的主要成分，但被认为是人类致癌物，并具有神经毒性。怀孕期间饮酒通常会导致胎儿酒精综合症。 DNA损伤是致癌性或致畸性的重要因素之一。为了检测乙醇诱导的DNA损伤，我们在怀孕的小鼠器官和胚胎中使用了体内碱性单细胞凝胶电泳（Comet）分析。妊娠第7天的妊娠ICR小鼠用2、4、8克/千克乙醇处理，在乙醇处理后的4、8、12和24小时，对母体器官/组织和胚胎进行彗星试验​​。在处理后的4或8小时，有4 g / kg和8 g / kg的乙醇诱导了脑，肺和胚胎的DNA损伤。 2 g / kg乙醇不会引起任何DNA损伤，而8 g / kg乙醇只会增加DNA损伤的持续时间，而损伤程度没有明显增加。在肝脏中未观察到明显的DNA损伤。为了检测乙醛的作用，在4 g / kg乙醇处理之前先施用乙磺胺乙醛脱氢酶抑制剂双硫仑。在双硫仑预处理组中未观察到DNA损伤的显着增加。这些数据表明乙醇诱导DNA损伤，这可能与乙醇毒性有关。由于双硫仑的预处理不会增加DNA损伤，因此本研究中观察到的DNA损伤可能不是乙醛的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号