Detection of in vivo genotoxicity of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) by the alkaline single cell gel electrophoresis (Comet) assay in multiple mouse organs.

Sasaki YF; Nishidate E; Izumiyama F; Watanabe Akanuma M; Kinae N; Matsusaka N; Tsuda S

首页> 外文期刊>Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects >Detection of in vivo genotoxicity of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) by the alkaline single cell gel electrophoresis (Comet) assay in multiple mouse organs.

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Detection of in vivo genotoxicity of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) by the alkaline single cell gel electrophoresis (Comet) assay in multiple mouse organs.

机译：通过碱性单细胞凝胶电泳（Comet）测定在多个小鼠器官中检测3-氯-4-（二氯甲基）-5-羟基-2（5H）-呋喃酮（MX）的体内遗传毒性。

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摘要

We tested the genotoxicity of 3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-furanone (MX) in the mouse in 6 organs (liver, lung, kidney, brain, spleen, and bone marrow) and in the mucosa of stomach, jejunum, ileum, colon, and bladder using the alkaline single-cell gel electrophoresis (SCG) (Comet) assay modified by us. Mice were sacrificed 1, 3, 6, and 24 h after oral administration of the mutagen at 100 mg/kg. MX yielded statistically significant DNA damage in the liver, kidney, lung, and brain and in all the mucosa samples. While DNA damage persisted in the gastrointestinal and urinary tract for 6-24 h after a single oral dosing, it peaked in the liver at 1 h and returned to almost the control level at 3 h. Our present results suggest that MX is genotoxic for various mouse organs, but not for the hematopoietic system, and that the alkaline SCG assay with a homogenization technique can be used to predict genotoxicity in the gastrointestinal and urinary tracts.

机译：我们在小鼠的6个器官（肝，肺，肾，脑，脾和骨髓）中测试了3-氯-4-（二氯甲基）-5-羟基-2 [5H]-呋喃酮（MX）的遗传毒性，并使用我们改良的碱性单细胞凝胶电泳（SCG）（Comet）分析方法在胃，空肠，回肠，结肠和膀胱的黏膜中进行检测。口服100 mg / kg诱变剂后1、3、6和24 h处死小鼠。 MX在肝，肾，肺和脑以及所有粘膜样品中产生统计上显着的DNA损伤。单次口服给药后，DNA损伤在胃肠道和泌尿道中持续存在6-24小时，但在1小时后在肝脏中达到峰值，并在3小时后恢复到几乎对照组的水平。我们目前的研究结果表明MX对多种小鼠器官具有遗传毒性，但对造血系统却无毒性，并且采用均质技术的碱性SCG分析法可用于预测胃肠道和泌尿道的遗传毒性。

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来源
《Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects》 |1997年第2期|共7页
作者
Sasaki YF; Nishidate E; Izumiyama F; Watanabe Akanuma M; Kinae N; Matsusaka N; Tsuda S;
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正文语种 eng
中图分类基础医学;
关键词
Furans; Mutagenicity Tests; Mutagens; 呋喃类; 诱变力试验; 诱变剂;

机译：Furans;Mutagenicity Tests;Mutagens;呋喃类;诱变力试验;诱变剂;

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1. Detection of in vivo genotoxicity of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) by the alkaline single cell gel electrophoresis (Comet) assay in multiple mouse organs. [J] . Sasaki YF, Nishidate E, Izumiyama F, Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 1997,第1a2期

机译：通过碱性单细胞凝胶电泳（Comet）测定在多个小鼠器官中检测3-氯-4-（二氯甲基）-5-羟基-2（5H）-呋喃酮（MX）的体内遗传毒性。
2. Detection of rodent liver carcinogen genotoxicity by the alkaline single-cell gel electrophoresis (Comet) assay in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow). [J] . Sasaki YF, Izumiyama F, Nishidate E, Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 1997,第3期

机译：通过碱性单细胞凝胶电泳（Comet）测定在多个小鼠器官（肝，肺，脾，肾和骨髓）中检测啮齿类动物肝癌的遗传毒性。
3. Colon-specific genotoxicity of heterocyclic amines detected by the modified alkaline single cell gel electrophoresis assay of multiple mouse organs. [J] . Sasaki YF, Saga A, Yoshida K, Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 1998,第1a3期

机译：通过修饰的多个小鼠器官的碱性单细胞凝胶电泳分析法检测到的杂环胺的结肠特异性遗传毒性。
4. Pilot-plant study of the formaiton of the disinfection by-product 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) in dinking water [C] . C.J.Hwang, S.W.Krasner, S.A.Andrews, Ozonation for drinking water treatment . 1992

机译：在饮用水中形成消毒副产物3-氯-4-（氯甲基）-5-羟基-2（5H）-呋喃酮（MX）的中试研究
5. Determination of genotoxic stress in feral populations of bullheads and carp using the alkaline single cell gel assay. [D] . Pandrangi, Raj Gopal. 1997

机译：使用碱性单细胞凝胶测定法测定牛头和鲤鱼野生种群的遗传毒性胁迫。
6. 3-Chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) and mutagenic activity in Massachusetts drinking water. [O] . J Michael Wright, Joel Schwartz, Terttu Vartiainen, 2002

机译：马萨诸塞州饮用水中的3-Chloro-4-（dichloromethyl）-5-hydroxy-2（5H）-呋喃酮（MX）和诱变活性。
7. Detection of in vivo DNA Damage Induced by Ethanol in Multiple Organs of Pregnant Mice Using the Alkaline Single Cell Gel Electrophoresis (Comet) Assay [O] . Ryoko KIDO, Itaru SATO, Shuji TSUDA 2006

机译：使用碱性单细胞凝胶电泳（COMET）测定，检测乙醇诱导的乙醇诱导的乙醇诱导的乙醇损伤
8. Induction of Gastrointestinal Tract Nuclear Anomalies in B6C3F1 Mice by 3-Chloro-4-(Dichloromethyl)-5-Hydroxy-2(5H)-Furanone and 3,4-(Dichloro)-5-Hydroxy-2(5H)-Furanone, Mutagenic Byproducts of Chlorine Disinfection [R] . Daniel, F. B., Olson, G. R., Stober, J. A. 1991

机译：用3-氯-4-（二氯甲基）-5-羟基-2（5H） - 呋喃酮和3,4-（二氯）-5-羟基-2（5H） - 呋喃酮诱导B6C3F1小鼠胃肠道核异常，氯消毒的诱变副产物

Detection of in vivo genotoxicity of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) by the alkaline single cell gel electrophoresis (Comet) assay in multiple mouse organs.

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