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首页> 外文期刊>The Korean Journal of Physiology & Pharmacology >Diversity of Ion Channels in Human Bone Marrow Mesenchymal Stem Cells from Amyotrophic Lateral Sclerosis Patients
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Diversity of Ion Channels in Human Bone Marrow Mesenchymal Stem Cells from Amyotrophic Lateral Sclerosis Patients

机译：肌萎缩侧索硬化患者骨髓间充质干细胞中离子通道的多样性

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摘要

Human bone marrow mesenchymal stem cells (hBM-MSCs) represent a potentially valuable cell type for clinical therapeutic applications. The present study was designed to evaluate the effect of long-term culturing (up to 10th passages) of hBM-MSCs from eight individual amyotrophic lateral sclerosis (ALS) patients, focusing on functional ion channels. All hBM-MSCs contain several MSCs markers with no significant differences, whereas the distribution of functional ion channels was shown to be different between cells. Four types of K+ currents, including noise-like Ca+2-activated K+ current (IKCa), a transient outward K+ current (Ito), a delayed rectifier K+ current (IKDR), and an inward-rectifier K+ current (Kir) were heterogeneously present in these cells, and a TTX-sensitive Na+ current (INa,TTX) was also recorded. In the RT-PCR analysis, Kv1.1 , heag1 , Kv4.2 , Kir2.1 , MaxiK , and hNE-Na were detected. In particular, INa,TTX showed a significant passage-dependent increase. This is the first report showing that functional ion channel profiling depend on the cellular passage of hBM-MSCs
机译：人骨髓间充质干细胞（hBM-MSC）代表了潜在的有价值的细胞类型，可用于临床治疗。本研究旨在评估来自八名肌萎缩性侧索硬化症（ALS）患者的hBM-MSC的长期培养（最多10次传代）的效果，重点是功能性离子通道。所有的hBM-MSC都包含多个MSCs标记，无明显差异，而细胞之间功能性离子通道的分布却有所不同。四种类型的K + 电流，包括类似噪声的Ca +2 激活的K + 电流（IK Ca ），瞬时向外K + 电流（I to ），延迟整流器K + 电流（IK DR ），并且在这些电池中异质地存在内向整流器K + 电流（K ir ），以及对TTX敏感的Na + 电流（I Na，TTX ）也被记录下来。在RT-PCR分析中，检测到Kv1.1，heag1，Kv4.2，Kir2.1，MaxiK和hNE-Na。特别是，I Na，TTX 表现出显着的传代依赖性增加。这是第一个报告，表明功能性离子通道谱取决于hBM-MSC的细胞传代

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