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首页> 外文期刊>The Korean Journal of Physiology & Pharmacology >Testosterone Relaxes Rabbit Seminal Vesicle by Calcium Channel Inhibition
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Testosterone Relaxes Rabbit Seminal Vesicle by Calcium Channel Inhibition

机译：睾丸激素通过钙通道抑制作用松弛兔精囊

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Recent studies have documented that testosterone relaxes several smooth muscles by modulating K+ channel activities. Smooth muscles of seminal vesicles play a fundamental role in ejaculation, which might involve testosterone. This study was aimed to assess the role of testosterone in seminal vesicular motility by studying its effects on contractile agents and on the ion channels of single vesicular myocytes in a rabbit model. The contractile responses of circular smooth muscle strips of rabbit seminal vesicles to norepinephrine (10 μM), a high concentration of KCl (70 mM), and testosterone (10 μM) were observed. Single vesicular myocytes of rabbit were isolated using proteolytic enzymes including collagenase and papain. Inside-out, attached, and whole-cell configurations were examined using the patch clamp technique. The applications of 10 μM norepinephrine or 70 mM KCl induced tonic contractions, and 10 μM testosterone (pharmacological concentration) evoked dose-dependent relaxations of these precontracted strips. Various K+ channel blockers, such as tetraethylammonium (TEA; 10 mM), iberiotoxin (0.1 μM), 4-aminopyridine (4-AP, 10 μM), or glibenclamide (10 μM) rarely affected these relaxations. Single channel data (of inside-out and attached configurations) of BK channel activity were also hardly affected by testosterone (10 μM). On the other hand, however, testosterone reduced L-type Ca2+ currents significantly, and found to induce acute relaxation of seminal vesicular smooth muscle and this was mediated, at least in part, by Ca2+ current inhibition in rabbit.
机译：最近的研究表明，睾丸激素可以通过调节K + 通道的活动来放松几条平滑肌。精囊的平滑肌在射精中起基本作用，可能涉及睾丸激素。本研究旨在通过研究睾丸激素对收缩剂和兔模型中单个囊泡心肌细胞离子通道的影响，来评估睾丸激素在精囊运动中的作用。观察到兔精囊的圆形平滑肌条对去甲肾上腺素（10μM），高浓度的KCl（70 mM）和睾丸激素（10μM）的收缩反应。使用包括胶原酶和木瓜蛋白酶的蛋白水解酶分离兔的单个囊泡心肌细胞。使用膜片钳技术检查了由内而外，附着的和全细胞配置。 10μM去甲肾上腺素或70 mM KCl引起的强直性收缩和10μM睾丸激素（药理学浓度）引起这些预收缩试纸的剂量依赖性松弛。各种K + 通道阻滞剂，例如四乙铵（TEA; 10 mM），埃博毒素（0.1μM），4-氨基吡啶（4-AP，10μM）或格列苯脲（10μM）很少影响这些放松。 BK通道活性的单通道数据（由内而外和附着的构型）也几乎不受睾丸激素（10μM）的影响。然而，另一方面，睾丸激素显着降低了L型Ca 2 + 电流，并发现其诱导精囊平滑肌急性松弛，这至少部分地由Ca 2 + 电流抑制

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