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首页> 外文期刊>The Journal of Reproduction and Development >The Effect of Single Nucleotide Polymorphisms in the Tumor Necrosis Factor-α Gene on Reproductive Performance and Immune Function in Dairy Cattle
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The Effect of Single Nucleotide Polymorphisms in the Tumor Necrosis Factor-α Gene on Reproductive Performance and Immune Function in Dairy Cattle

机译：肿瘤坏死因子-α基因单核苷酸多态性对奶牛繁殖性能和免疫功能的影响

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The present study aimed to assess the effect of polymorphisms in the tumor necrosis factor α (TNF-α) promoter (A/A, A/G and G/G) and exons (T/T, T/C and C/C) on immune function and reproductive performance in dairy cows. The occurrence of the first postpartum ovulation within 3 weeks in the cows with the TNF-α promoter A/G and G/G genotypes was higher than in the A/A group. Among the different TNF-α exon genotypes, the occurrence of early first postpartum ovulation was higher in the T/C and C/C genotype groups than in the T/T group. Single nucleotide polymorphisms (SNPs) in the TNF-α gene did not affect the rate of artificial insemination (AI) or duration from parturition to next conception (days open). The apoptosis rate of polymorphonuclear leukocytes (PMNs) did not differ among the TNF-α promoter genotypes, but the PMN transmigration rate was significantly higher for the A/A and A/G genotypes than for the G/G genotype. Interleukin 8 (IL-8) mRNA expression in PMNs and peripheral blood mononuclear cells (PBMCs) before culture was significantly higher for the A/A genotype compared with the G/G genotype. There were no significant differences between the genotypes in the mRNA expression of TNF-α, IL-6, IL-1β, and toll-like receptor 4 (TLR4) in PMNs and PBMCs before and 4 h after culture. IL-8 and IL-1β production by PBMCs cultured for 4 h was significantly higher for the animals with the A/A genotype than for those with the G/G genotype. On the other hand, no significant difference was observed in IL-8 and IL-1β production by PMNs among different TNF-α genotypes. Taken together, these results suggest that SNP in the TNF-α gene affects immune function and reproductive performance in dairy cows.
机译：本研究旨在评估多态性对肿瘤坏死因子α（TNF-α）启动子（A / A，A / G和G / G）和外显子（T / T，T / C和C / C）的影响对奶牛的免疫功能和生殖性能的影响。具有TNF-α启动子A / G和G / G基因型的母牛在3周内第一次产后排卵的发生率高于A / A组。在不同的TNF-α外显子基因型中，T / C和C / C基因型组的首次产后早期排卵发生率高于T / T组。 TNF-α基因中的单核苷酸多态性（SNP）不会影响人工授精（AI）的速度或从分娩到下一个受孕的持续时间（开放日）。在TNF-α启动子基因型之间，多形核白细胞（PMN）的凋亡率没有差异，但是A / A和A / G基因型的PMN迁移率显着高于G / G基因型。与G / G基因型相比，A / A基因型在培养前PMN和外周血单个核细胞（PBMC）中的白细胞介素8（IL-8）mRNA表达显着更高。在培养前和培养后4 h，PMN和PBMC中TNF-α，IL-6，IL-1β和toll样受体4（TLR4）的mRNA表达在基因型之间没有显着差异。具有A / A基因型的动物培养4 h的PBMC产生的IL-8和IL-1β明显高于具有G / G基因型的动物。另一方面，在不同的TNF-α基因型之间，PMN产生的IL-8和IL-1β没有显着差异。综上所述，这些结果表明TNF-α基因中的SNP影响奶牛的免疫功能和生殖性能。

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