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首页> 外文期刊>The Journal of Reproduction and Development >Localization of a Novel RNA-binding Protein, SKIV2L2, to the Nucleus in the Round Spermatids of Mice
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Localization of a Novel RNA-binding Protein, SKIV2L2, to the Nucleus in the Round Spermatids of Mice

机译：新型RNA结合蛋白SKIV2L2，定位到小鼠圆形精子细胞核中的定位。

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In our previous study (Kawashima et al., Biol Reprod 2009; 80: 1293-1304), we suggested that the first cycle of spermatogenesis recovered from busulfan-induced temporary arrest was a good model system to analyze the proteins expressed at the specific stages of spermatogenesis in the mouse, and this has been confirmed in the present paper. Namely, six-week-old mice were injected with busulfan at 20 mg/kg body weight. The germ cells except for the undifferentiated spermatogonia disappeared by 32 days after injection. The surviving spermatogonia started to proliferate, and spermatogenesis was entirely recovered about 77 days after injection. By proteome analysis of the busulfan-treated testis during the process of recovery of spermatogenesis, we identified a protein that was expected to be expressed in the spermatogenic cells as Superkiller viralicidic activity-2-like-2 (SKIV2L2). Skiv2l2 mRNA was found in the kidney, epididymis and heart as well as the testis. In the testis, Skiv2l2 mRNA was shown to be highly expressed in the spermatocytes at stages I to VI. On the other hand, SKIV2L2 protein was found to be predominantly localized in the nuclei of round spermatids. In accordance with the fact that SKIV2L2 belongs to the Ski2 family within the superfamily 2 of RNA helicases, it has been shown that SKIV2L2 has both the RNA-binding and ATPase activities.
机译：在我们之前的研究中（Kawashima等人，Biol Reprod 2009; 80：1293-1304），我们认为从白消安诱导的暂时性阻滞中恢复的精子发生的第一个周期是分析特定阶段表达的蛋白质的良好模型系统小鼠精子发生的机理，这一点已在本论文中得到证实。即，给六周大的小鼠注射了20mg / kg体重的白消安。注射后32天，除未分化的精原细胞外的生殖细胞消失。存活的精原细胞开始增生，注射后约77天精子发生完全恢复。通过在精子发生恢复过程中对白消硫处理过的睾丸进行蛋白质组分析，我们确定了预期在精子发生细胞中表达的蛋白质为Superkiller病毒杀伤活性类似2（SKIV2L2）。 Skiv2l2 mRNA在肾脏，附睾，心脏以及睾丸中发现。在睾丸中，Skiv2l2 mRNA在I至VI阶段在精母细胞中高表达。另一方面，发现SKIV2L2蛋白主要定位在圆形精子的细胞核中。根据SKIV2L2属于RNA解旋酶超家族2的Ski2家族这一事实，已显示SKIV2L2同时具有RNA结合和ATPase活性。

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