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首页> 外文期刊>The Open Neurology Journal >The Lymphocyte Transformation Test for Borrelia Detects Active Lyme Borreliosis and Verifies Effective Antibiotic Treatment
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The Lymphocyte Transformation Test for Borrelia Detects Active Lyme Borreliosis and Verifies Effective Antibiotic Treatment

机译：疏螺旋体的淋巴细胞转化测试可检测活性莱姆疏螺旋体病并验证有效的抗生素治疗

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Borrelia-specific antibodies are not detectable until several weeks after infection and even if they are present, they are no proof of an active infection. Since the sensitivity of culture and PCR for the diagnosis or exclusion of borreliosis is too low, a method is required that detects an active Borrelia infection as early as possible. For this purpose, a lymphocyte transformation test (LTT) using lysate antigens of Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii and recombinant OspC was developed and validated through investigations of seronegative and seropositive healthy individuals as well as of seropositive patients with clinically manifested borreliosis. The sensitivity of the LTT in clinical borreliosis before antibiotic treatment was determined as 89,4% while the specificity was 98,7%. In 1480 patients with clinically suspected borreliosis, results from serology and LTT were comparable in 79.8% of cases. 18% were serologically positive and LTT-negative. These were mainly patients with borreliosis after antibiotic therapy. 2.2% showed a negative serology and a positive LTT result. Half of them had an early erythema migrans. Following antibiotic treatment, the LTT became negative or borderline in patients with early manifestations of borreliosis, whereas in patients with late symptoms, it showed a regression while still remaining positive. Therefore, we propose the follow-up monitoring of dis-seminated Borrelia infections as the main indication for the Borrelia-LTT.
机译：直到感染后几周才检测到疏螺旋体特异性抗体，即使它们存在也不能证明是主动感染。由于培养和PCR诊断或排除疏螺旋体病的敏感性太低，因此需要一种方法来尽早检测活动性疏螺旋体感染。为此，通过对血清阴性和血清阳性的健康个体以及临床表现为硼肺病的血清阳性和血清阳性患者的研究，开发了使用伯氏疏螺旋体，严格的疏螺旋体和细疏疏螺旋体的裂解物抗原的淋巴细胞转化试验（LTT），并进行了验证。 。在抗生素治疗之前，LTT在临床性贝氏体病中的敏感性被确定为89,4％，而特异性为98,7％。在1480例临床怀疑的贝氏体病患者中，血清学和LTT结果在79.8％的病例中相当。 18％的血清学阳性和LTT阴性。这些患者主要是抗生素治疗后出现的贝氏体病。 2.2％的患者血清学检查阴性，LTT结果阳性。他们中的一半患有早期红斑偏头痛。抗生素治疗后，在早期表现为疏螺旋体病的患者中，LTT变为阴性或临界值，而在晚期症状的患者中，LTT呈下降趋势，而仍保持阳性。因此，我们建议对散播的疏螺旋体感染进行随访监测，作为疏螺旋体-LTT的主要指征。

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