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首页> 外文期刊>The Open Obesity Journal >Effect of a High-Fat Diet on 24-Hour Pattern in Expression of Prolactin and Redox Pathway Enzymes in the Rat Adenohypophysis
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Effect of a High-Fat Diet on 24-Hour Pattern in Expression of Prolactin and Redox Pathway Enzymes in the Rat Adenohypophysis

机译：高脂饮食对大鼠腺垂体催乳素和氧化还原途径酶表达的24小时模式的影响

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The effect of a high-fat diet (35% fat) on 24-h changes in expression of prolactin and redox pathway enzyme genes was examined in the anterior pituitary of male rats. When body weight of high-fat fed rats attained values about 20- 25 % higher than controls (after 66 days of treatment) animals were sacrificed at 6 different time intervals throughout a 24-h cycle. Anterior pituitary mRNA levels were measured by real-time PCR analysis. In control rats expression of the prolactin gene peaked in anterior pituitary at early scotophase, 3 h in advance to the peak in plasma prolactin levels. In high-fat fed rats this correlation was lost. A disruption of 24-h rhythmicity of pituitary gene expression of heme oxygenase (HO)-2, Cu/Zn- superoxide dismutases (SOD), Mn-SOD, catalase, glutathione peroxidase (GPx) and glutathione reductase (GR) was apparent in high-fat fed rats. Anterior pituitary mRNA levels for nitric oxide synthase (NOS)-2 and HO-2, Cu/Zn- and Mn- SOD and catalase augmented in high-fat fed rats, whereas those of GPx and GR decreased. The results indicate a disrupted coordination between prolactin gene expression and prolactin release in highfat fed rats that comes along with a disturbed expression of redox enzyme genes in the anterior pituitary.
机译：在雄性大鼠垂体前叶中检查了高脂饮食（35％的脂肪）对催乳素和氧化还原途径酶基因表达24小时变化的影响。当高脂喂养的大鼠的体重达到比对照高约20％至25％的值时（治疗66天后），在整个24小时的周期中以6个不同的时间间隔处死动物。垂体前叶mRNA水平通过实时PCR分析测量。在对照大鼠中，催乳素基因的表达在早于蜕皮期的垂体前叶达到峰值，比血浆催乳素水平提前3小时。在高脂喂养的大鼠中，这种相关性消失了。血红素加氧酶（HO）-2，Cu / Zn-超氧化物歧化酶（SOD），Mn-SOD，过氧化氢酶，谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GR）的垂体基因表达的24小时节律性破坏明显。高脂喂养的大鼠。一氧化氮合酶（NOS）-2和HO-2，Cu / Zn和Mn- SOD和过氧化氢酶的垂体前叶mRNA水平在高脂喂养大鼠中增加，而GPx和GR则下降。结果表明在高脂喂养的大鼠中催乳素基因表达和催乳素释放之间的协调受到破坏，同时垂体前叶氧化还原酶基因表达受到干扰。

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