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首页> 外文期刊>The Veterinary Quarterly >Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection
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Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection

机译：通过MALDI-TOF质谱鉴定和从母猪尿路感染中分离出的细菌病原体的抗菌药敏分析

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Background: Urinary tract infection (UTI) is a common disease in sows due to intensification of pig production. Despite direct economic losses, UTI prevalence and respective microbial identification are still poorly studied.Objective: The aims of this study were to identify the causative agents of UTI in sows through MALDI-TOF MS and to characterize their antimicrobial resistance profiles.Materials and Methods: Urine samples from 300 sows of three herds from S?o Paulo State (Brazil) were screened for UTI; suggestive samples were submitted to bacterial isolation. Species identification was performed by MALDI-TOF MS and susceptibility profiles were determined using disc diffusion method.Results: 128 samples suggestive of UTI were analyzed; 48% of the animals presented UTI caused by a single pathogen, while the remaining 52% presented mixed infection. Escherichia coli stood out with the highest frequency among both single and mixed infections. The Gram-positive were exclusively associated with 27% of single infections. The mixed infections were further classified into 49 profiles. The high frequency of multiresistant profiles stood out for most of the studied isolates.Conclusions: MALDI-TOF MS enabled the identification of rare pathogens related to UTI which may represent higher risk for porcine health, especially considering high frequency of multiresistant profiles.
机译：背景：泌尿道感染（UTI）是母猪的常见疾病，原因是猪的生产增加。尽管存在直接的经济损失，但对UTI的发生率和有关微生物的鉴定仍然缺乏研究。目的：本研究的目的是通过MALDI-TOF MS鉴定母猪中UTI的病原体并表征其抗药性概况。对来自圣保罗州（巴西）的三只猪群的300头母猪的尿液样本进行了尿道感染检测;建议将样本进行细菌分离。通过MALDI-TOF MS进行物种鉴定，并采用圆盘扩散法测定药敏图谱。结果：分析了128例提示UTI的样品。 48％的动物呈现由单一病原体引起的UTI，其余52％的动物呈现混合感染。在单一和混合感染中，大肠杆菌的发病率最高。革兰氏阳性仅与27％的单一感染相关。混合感染被进一步分为49种。结论：MALDI-TOF MS能够鉴定与UTI相关的罕见病原体，这些病原体可能代表猪的健康风险更高，特别是考虑到多耐药谱的高发生率。

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