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首页> 外文期刊>The Journal of Food >DETERMINATION OF FLAVONOIDS IN CITRUS FIBER AND THE IMPACT OF PROCESSING METHOD ON TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, TOTAL CAROTENOID CONTENT AND OXYGEN RADICAL ABSORBANCE CAPACITY
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DETERMINATION OF FLAVONOIDS IN CITRUS FIBER AND THE IMPACT OF PROCESSING METHOD ON TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, TOTAL CAROTENOID CONTENT AND OXYGEN RADICAL ABSORBANCE CAPACITY

机译：柑橘纤维中黄酮含量的测定及其处理方法对总酚含量，总黄酮含量，总类胡萝卜素含量和氧自由基吸收能力的影响

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摘要

The objective of this study is to evaluate the presence of flavonoids in citrus f?ber using reverse-phase high-pressure liquid chromatography (RP-HPLC). In this study, we also investigate the effect of regular and hot-washing preparations on total polyphenol content (TPC), total flavonoid content (TFC), oxygen radical absorbance capacity (ORAC) and total carotenoid content (TCC) of citrus fiber. The HPLC method used provided fast results and good separation for polymethoxyflavones and flavonols. Quercetin and kaempferol (flavonols), and sinensetin, nobiletin, heptamethoxyflavone, and tangeretin (polymethoxyflavones) were identified. Nobiletin had the highest concentration with 3.33 mg/g dry weight followed by sinensetin (1.96 mg/g dry weight) and heptamethoxyflavone (1.24 mg/g dry weight), respectively. The TFC and TCC of citrus fiber were unaffected (P >0.05) by the citrus fiber production procedure; however, the TPC and the ORAC values of citrus fiber prepared using hot-washing were significantly lower (P < 0.05).
机译：这项研究的目的是使用反相高压液相色谱法（RP-HPLC）评估柑橘类纤维中黄酮类化合物的存在。在这项研究中，我们还研究了常规和热洗制剂对柑桔纤维总多酚含量（TPC），总黄酮含量（TFC），氧自由基吸收能力（ORAC）和总类胡萝卜素含量（TCC）的影响。所用的HPLC方法对聚甲氧基黄酮和黄酮醇提供了快速的结果和良好的分离效果。鉴定出了槲皮素和山ol酚（黄酮醇）以及芥子素，诺比列汀，七甲氧基黄酮和橘皮素（聚甲氧基黄酮）。 Nobiletin的最高浓度为3.33 mg / g干重，其次是芥子素（1.96 mg / g干重）和七甲氧基黄酮（1.24 mg / g干重）。柑橘纤维的生产程序不影响柑橘纤维的TFC和TCC（P> 0.05）;然而，热洗法制得的柑橘纤维的TPC和ORAC值明显较低（P <0.05）。

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