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首页> 外文期刊>The open biotechnology journal >Enhanced Interferon-α2b Production in Periplasmic Space of Escherichia coli through Medium Optimization using Response Surface Method
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Enhanced Interferon-α2b Production in Periplasmic Space of Escherichia coli through Medium Optimization using Response Surface Method

机译：通过响应面法优化培养基，提高了大肠杆菌周质空间中干扰素-α2b的产量

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The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and humaninterferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method basedon Plackett-Burman design was used to screen the main medium components that greatly influenced the performance ofthe fermentation process. The optimization of medium was performed using response surface methodology (RSM) wherethree critical factors (glucose, yeast extract and peptone) were optimized using central composite design. The highest yieldof periplasmic recombinant human interferon-α2b (PrIFN-α2b) (335.8 μg/L) was predicted to be obtained in optimizedmedium containing 5.47 g/L glucose, 55.24 g/L yeast extract and 42.27 g/L peptone.. The production of IFN-α2b in periplasmicspace in optimized medium was about 2.5, 11.7 and 124.4 times higher than Terrific broth (TB), Luria-Bertani(LB), and minimal medium (M9), respectively.
机译：在摇瓶培养中研究了碳源和氮源对重组大肠杆菌生长和周质空间人干扰素-α2b（IFN-α2b）产生的影响。使用基于Plackett-Burman设计的统计方法来筛选对发酵过程的性能有重大影响的主要培养基成分。使用响应表面方法（RSM）进行培养基的优化，其中使用中心复合设计优化了三个关键因素（葡萄糖，酵母提取物和蛋白ept）。预测在含有5.47 g / L葡萄糖，55.24 g / L酵母提取物和42.27 g / L蛋白ept的优化培养基中，可获得最高产量的质体重组人干扰素-α2b（PrIFN-α2b）（335.8μg/ L）。优化培养基中周质空间中IFN-α2b的含量分别比Terrific肉汤（TB），Luria-Bertani（LB）和基本培养基（M9）高约2.5、11.7和124.4倍。

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