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首页> 外文期刊>Toxins >Ameliorating Effects of Bacillus subtilis ANSB060 on Growth Performance, Antioxidant Functions, and Aflatoxin Residues in Ducks Fed Diets Contaminated with Aflatoxins
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Ameliorating Effects of Bacillus subtilis ANSB060 on Growth Performance, Antioxidant Functions, and Aflatoxin Residues in Ducks Fed Diets Contaminated with Aflatoxins

机译:枯草芽孢杆菌ANSB060对鸭饲料中黄曲霉毒素污染的生长性能,抗氧化功能和黄曲霉毒素残留的改善作用

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摘要

Bacillus subtilis ANSB060 isolated from fish gut is very effective in detoxifying aflatoxins in feed and feed ingredients. The purpose of this research was to investigate the effects of B. subtilis ANSB060 on growth performance, body antioxidant functions, and aflatoxin residues in ducks fed moldy maize naturally contaminated with aflatoxins. A total of 1500 18-d-old male Cherry Valley ducks with similar body weight were randomly assigned to five treatments with six replicates of 50 ducks per repeat. The experiment design consisted of five dietary treatments labeled as C0 (basal diet containing 60% normal maize), M0 (basal diet containing 60% moldy maize contaminated with aflatoxins substituted for normal maize), M500, M1000, and M2000 (M0 +500, 1000 or 2000 g/t aflatoxin biodegradation preparation mainly consisted of B. subtilis ANSB060). The results showed that ducks fed 22.44 ± 2.46 μg/kg of AFB 1 (M0) exhibited a decreasing tendency in average daily gain (ADG) and total superoxide dismutase (T-SOD) activity in serum, and T-SOD and glutathione peroxidase (GSH-Px) activities in the liver significantly decreased along with the appearance of AFB 1 and AFM 1 compared with those in Group C0. The supplementation of B. subtilis ANSB060 into aflatoxin-contaminated diets increased the ADG of ducks ( p > 0.05), significantly improved antioxidant enzyme activities, and reduced aflatoxin accumulation in duck liver. In conclusion, Bacillus subtilis ANSB060 in diets showed an ameliorating effect to duck aflatoxicosis and may be a promising feed additive.
机译:从鱼肠中分离出的枯草芽孢杆菌ANSB060在排毒饲料和饲料成分中的黄曲霉毒素方面非常有效。这项研究的目的是调查枯草芽孢杆菌ANSB060对饲喂天然被黄曲霉毒素污染的发霉玉米的鸭的生长性能,身体抗氧化功能和黄曲霉毒素残留的影响。将总共​​1500只体重相似的18日龄雄性樱桃谷野鸭随机分配到5种处理中,每重复6次,每只重复50只。实验设计包括五种饮食处理,分别标记为C0(基本日粮中含有60%正常玉米),M0(基本日粮中含有60%发霉的玉米并污染了黄曲霉毒素,替代了正常玉米),M500,M1000和M2000(M0 +500, 1000或2000 g / t黄曲霉毒素的生物降解制剂,主要由枯草芽孢杆菌(ANSB060)组成。结果表明,喂食22.44±2.46μg/ kg的AFB 1(M0)的鸭子的血清平均日增重(ADG)和总超氧化物歧化酶(T-SOD)活性以及T-SOD和谷胱甘肽过氧化物酶(与C0组相比,肝脏中的GSH-Px)活性以及AFB 1和AFM 1的出现显着降低。在黄曲霉毒素污染的日粮中添加枯草芽孢杆菌ANSB060可提高鸭的ADG(p> 0.05),显着提高抗氧化酶的活性,并减少鸭肝中黄曲霉毒素的积累。总之,日粮中的枯草芽孢杆菌ANSB060对鸭肉中毒有改善作用,可能是有前途的饲料添加剂。

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