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首页> 外文期刊>Tropical Journal of Pharmaceutical Research >Protective Effects of Dimedone Pyrone on Podocytes in Rats with Diabetic Nephropathy
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Protective Effects of Dimedone Pyrone on Podocytes in Rats with Diabetic Nephropathy

机译：二甲酮吡喃酮对糖尿病肾病大鼠足细胞的保护作用

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Purpose: To investigate the effect of dimedone pyrone (DP) on podocytes in rats with diabetic nephropathy (DN). Methods: The rats were randomly assigned into 5 experimental groups (n = 10), viz, non-diabetic control with no treatment (ND/NT), diabetic with no treatment (DG/NT), diabetic treated with 5 mg/kg dimedone pyrone (DG/DP 5), diabetic treated with 10 mg/kg dimedone pyrone (DG/DP 10) and diabetic treated with 20 mg/kk dimedone pyrone (DG/DP 20) group. Clinical parameters, including 24 h urinary protein, blood urea nitrogen (BUN), serum creatinine (SCR), blood glucose (GLU), and kidney weight (KW)/body weight (BW) were determined after 12 weeks of treatment. Hematoxylin and eosin staining was used to examine renal pathological changes while transmission electron microscopy (TEM) was employed for evaluation of structural changes in the podocytes. The expression levels of nephrin and podocin were evaluated using immunofluorescence staining. Results: Dimedone pyrone caused a significant decrease in SCR, BUN, GLU, KW/BW and 24 h urine protein in DG/DP 20 group compared to DG/NT group. Furthermore, incidences of glomerular disorders, chronic tubulo-interstitial damage and glomerular podocyte lesions decreased significantly following dimedone pyrone treatment. Glomeruli, tubules and podocytes exhibited pathomorphological improvements while nephrin and podocin protein expression levels were significantly higher in the nephridial tissue. Decrease in relative kidney weight (KW/BW) and 24 h urinary protein level were improved significantly on treatment with dimedone pyrone. Moreover, glomerular disorder, chronic tubulo-interstitial damage and glomerular podocyte lesions were also suppressed. The improvement was more significant in DG/DP 20 compared to DG/DP 5 and DG/DP 10 groups. Conclusion: Dimedone pyrone exhibits a protective effect on the podocytes of rats and may be of therapeutic importance in the treatment of diabetic nephropathy.
机译：目的：研究二甲酮吡喃酮（DP）对糖尿病肾病（DN）大鼠足细胞的影响。方法：将大鼠随机分为5个实验组（n = 10），即，非糖尿病对照组，不治疗（ND / NT），糖尿病非治疗（DG / NT），糖尿病治疗，5 mg / kg二甲酮治疗吡喃酮（DG / DP 5），糖尿病患者用10 mg / kg二甲酮吡喃酮（DG / DP 10）治疗，糖尿病患者用20 mg / kk二甲酮吡喃酮（DG / DP 20）治疗。在治疗12周后确定临床参数，包括24小时尿蛋白，血尿素氮（BUN），血清肌酐（SCR），血糖（GLU）和肾脏重量（KW）/体重（BW）。苏木和曙红染色用于检查肾脏病理变化，而透射电镜（TEM）用于评估足细胞的结构变化。使用免疫荧光染色评估nephrin和podocin的表达水平。结果：与DG / NT组相比，DG / DP 20组的二甲酮吡喃可显着降低SCR，BUN，GLU，KW / BW和24 h尿蛋白。此外，二甲酮吡喃酮治疗后，肾小球疾病，慢性肾小管间质损害和肾小球足细胞病变的发生率显着降低。肾小球，肾小管和足细胞表现出病理形态学改善，而肾组织中肾素和podocin蛋白的表达水平明显更高。用二甲酮吡喃治疗可显着改善相对肾脏重量（KW / BW）和24 h尿蛋白水平的降低。此外，肾小球疾病，慢性肾小管间质损伤和肾小球足细胞病变也得到抑制。与DG / DP 5和DG / DP 10组相比，DG / DP 20中的改善更为显着。结论：二甲酮吡喃酮对大鼠的足细胞具有保护作用，对糖尿病肾病的治疗可能具有重要的治疗意义。

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