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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Early pregnancy diagnosis using a commercial ELISA test based on pregnancy-associated glycoproteins in Holstein-Friesian heifers and lactating cows
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Early pregnancy diagnosis using a commercial ELISA test based on pregnancy-associated glycoproteins in Holstein-Friesian heifers and lactating cows

机译：使用基于荷斯坦-弗里斯兰小母牛和泌乳牛的妊娠相关糖蛋白的商业ELISA试验进行早期妊娠诊断

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摘要

In this study, we aimed to investigate the efficacy of a commercial ELISA test kit for detecting pregnancy-associated glycoproteins (PAGs) in peripheral blood for early pregnancy diagnosis and compare plasma PAG levels during early pregnancy in both Holstein-Friesian heifers and lactating cows. A total of 231 plasma samples were collected from heifers and lactating cows on days 25, 28, and 32 after insemination. Pregnancies were confirmed 30 days after the collection of plasma samples. Plasma PAG levels were measured using a commercial ELISA test kit for diagnosing bovine pregnancy. The effects of examination date and animal status (heifers vs. lactating cows) on PAG levels in heifers and lactating cows were analyzed using two-way ANOVA. Plasma PAG levels were significantly higher in heifers than in lactating cows; levels also increased significantly with the date of examination, i.e. days 25, 28, and 32 for heifers (P > 0.001), but not for lactating cows (P > 0.05). Although the sensitivity, specificity, and accuracy of pregnancy diagnosis using the ELISA test were acceptable in both groups, the performance of the test was superior in pregnant heifers compared to lactating cows.
机译：在这项研究中，我们旨在调查商业ELISA测试试剂盒在早期妊娠诊断中检测外周血中与妊娠相关的糖蛋白（PAG）的功效，并比较荷斯坦-弗里斯兰小母牛和泌乳母牛早期妊娠期间血浆PAG的水平。在授精后第25、28和32天，从小母牛和泌乳母牛中收集了总共231个血浆样品。收集血浆样品后30天确认怀孕。使用用于诊断牛妊娠的商业ELISA测试试剂盒测量血浆PAG水平。使用双向ANOVA分析检查日期和动物状况（小母牛与泌乳母牛）对小母牛和泌乳母牛PAG水平的影响。小母牛的血浆PAG水平显着高于泌乳母牛。血清水平也随检查之日显着增加，即小母牛的第25、28和32天（P> 0.001），但泌乳母牛则没有（P> 0.05）。尽管两组均可以接受使用ELISA试验进行妊娠诊断的敏感性，特异性和准确性，但与母牛相比，在怀孕的小母牛中，该试验的性能更高。

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