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Circulation of Schmallenberg virus in Turkey, 2013

机译:2013年土耳其Schmallenberg病毒的发行量

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Schmallenberg virus (SBV) infection emerged in European domestic and wild ruminants in 2011. There is very limited information about the characterization of SBV isolates and the epidemiology of its infections in the rest of world, except for in European countries. We investigated the circulation of SBV in cattle herds in Central Anatolia, Turkey, in 2013. A total of 180 whole-blood samples were analyzed using real-time RT-PCR. The presence of SBV RNA was detected in 6 (3.3%) samples. For phylogenetic analysis and confirmation of real-time RT-PCR results, the S gene segment was amplified, sequenced, and compared to other segments. In addition, SBV-specific antibodies were detected in 87 (24.1%) of 360 sera using a virus neutralization test. In the S gene sequence analysis of four randomly selected samples, 98%-99% nucleotide identity was observed between our strains and SBVs isolated in European countries between 2011 and 2013. The results of this study indicate that SBV was in Turkey in 2013. Furthermore, the sequencing results suggest that it could be the same virus that is in European countries.
机译:Schmallenberg病毒(SBV)感染在2011年出现在欧洲的家养和野生反刍动物中。除欧洲国家外,关于SBV分离株的特征及其感染的流行病学的信息非常有限。我们在2013年调查了土耳其中部安纳托利亚牛群中SBV的循环。使用实时RT-PCR分析了总共180个全血样品。在6个(3.3%)样品中检测到SBV RNA的存在。为了进行系统发育分析和实时RT-PCR结果的确认,对S基因片段进行了扩增,测序和与其他片段的比较。此外,使用病毒中和测试在360个血清中的87个(24.1%)中检测到SBV特异性抗体。在四个随机选择的样本的S基因序列分析中,我们的菌株与2011年至2013年间在欧洲国家分离的SBV之间观察到98%-99%的核苷酸同一性。这项研究的结果表明SBV在2013年在土耳其。 ,测序结果表明它可能与欧洲国家的病毒相同。

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